COL4A6 Rabbit Polyclonal Antibody for WB, ELISA - Q14031

Core Product Specifications and Parameters

Biological Background: Collagen alpha-6(IV) (COL4A6) Function and Localization

• The COL4A6 gene encodes collagen alpha-6(IV) chain, a component of type IV collagen, the major structural component of basement membranes.
• Type IV collagen forms a 'chicken-wire' meshwork together with laminins, proteoglycans, and entactin/nidogen, providing structural support and filtration functions in glomerular basement membranes (GBM).
• COL4A6 belongs to the collagen IV gene family, which also includes COL4A1 to COL4A5, each contributing to the heterotrimeric collagen IV network.
• The protein is secreted into the extracellular space and is predominantly localized in the basement membrane, where it contributes to cell adhesion and matrix integrity.
• Post-translational modifications include hydroxylation and glycosylation, and the protein contains disulfide bonds and repeats typical of collagen chains.
• Alternative splicing of COL4A6 produces multiple isoforms, and mutations or chromosomal rearrangements involving this gene are associated with X-linked deafness (DFNX6).
• The protein has a predicted molecular weight of approximately 164 kDa and is identified in reference proteomes and proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fusion protein spanning amino acids 1575-1691 of human COL4A6, which may represent a unique region for specificity; consider validating reactivity in your specific sample matrices.
• This polyclonal antibody is suitable for WB and ELISA applications as indicated; for WB, consider using a molecular weight of 164 kDa for detection.
• Cross-reactivity with rat COL4A6 has been observed, allowing for potential use in rat models; confirm performance in your experimental system.
• For optimal results, titrate the antibody and include appropriate positive and negative controls, especially when analyzing tissues expressing COL4A6 such as kidney or extracellular matrix-rich samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing bridge for IVD raw materials, supporting your extracellular matrix and basement membrane research with a comprehensive portfolio of antibodies and ancillary reagents. Whether you need validated antibody pairs, bulk monoclonal/polyclonal antibodies, or expert technical support, CamelBio streamlines your procurement from concept to clinical application. Contact us to discuss your specific requirements for COL4A6-related assays.