COL2A1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02458

Core Product Specifications and Parameters

Biological Background: COL2A1 Function and Localization

• Collagen alpha-1(II) chain (COL2A1) is a major structural component of type II collagen, which is specifically found in cartilaginous tissues.
• It plays an essential role in normal embryonic skeletal development, linear growth, and provides cartilage with the ability to resist compressive forces.
• The protein is secreted and localized to the extracellular space and extracellular matrix.
• Alternative splicing produces isoform 2, which is highly expressed in juvenile chondrocytes but low in fetal chondrocytes.
• COL2A1 is associated with several diseases, including Stickler syndrome, skeletal dysplasias, and chondrodysplasias, as indicated by keywords such as disease variant, dwarfism, cataract, and deafness.
• Post-translational modifications include hydroxylation, glycosylation, and disulfide bond formation; it also binds calcium and metal ions.
• The mature protein has a molecular weight of approximately 141.8 kDa (UniProt: 141,785 Da) and contains a signal peptide for secretion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 1220-1282) of human COL2A1; consider using this region for validation in your sample system.
• For Western blotting (WB), the predicted molecular weight is ~142 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), due to the extracellular matrix localization, optimize antigen retrieval and permeabilization steps for cartilage tissue sections or chondrocyte cultures.
• When using ELISA, pairing with a matched detection antibody may improve assay sensitivity; validate with recombinant protein or known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive sourcing solution for IVD raw materials. This COL2A1 antibody supports investigations in cartilage biology, skeletal development, and related disorders. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and can source rare target raw materials to streamline your development pipeline.