CNTN4 Rabbit mAb for WB, IHC-P, ELISA - Q8IWV2

Core Product Specifications and Parameters

Biological Background: Contactin-4 Function and Localization

• Contactin-4 (CNTN4), also known as BIG-2 or AXCAM, is a member of the contactin family of immunoglobulin superfamily cell adhesion molecules.
• CNTN4 mediates cell surface interactions during nervous system development, exhibiting neurite outgrowth-promoting activity and potential involvement in synaptogenesis.
• The protein is localized to the cell membrane via a GPI anchor and can also be secreted; it contains immunoglobulin-like domains and disulfide bonds typical of the family.
• In humans, CNTN4 is mainly expressed in the brain, with high levels in the cerebellum and lower expression in the corpus callosum, caudate nucleus, amygdala, and spinal cord.
• Extra-neural expression is observed in testis, pancreas, thyroid, uterus, small intestine, and kidney, but not in skeletal muscle; isoform 2 shows weak expression in the cerebral cortex.
• Post-translational modifications include glycosylation and the presence of a GPI anchor, which are critical for its membrane association.
• CNTN4 is subject to alternative splicing, and chromosomal rearrangements involving its gene have been noted.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to a sequence within amino acids 800-900 of human CNTN4, so the antibody likely recognizes an epitope in this region; validate specificity using appropriate controls.
• For Western blotting (WB), the expected molecular weight is approximately 113 kDa; consider using proper protein extraction and loading controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution; validate in brain tissue where CNTN4 is highly expressed.
• In ELISA, this antibody can be used for detection or capture; pair with a suitable secondary antibody and confirm binding in relevant sample matrices.
• Because the antibody cross-reacts with human, mouse, and rat, it is suitable for comparative studies across these species; verify performance in each species' tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw-material sourcing, offering a comprehensive portfolio from concept to clinic. This anti-CNTN4 rabbit monoclonal antibody exemplifies our capability to supply high-quality reagents for neuroscience research and diagnostic development. Explore our range of validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing to accelerate your assay development with reliable components.