CNAP1 Rabbit mAb for WB and ELISA - Q15021

Core Product Specifications and Parameters

Biological Background: Condensin Complex Subunit 1 (CNAP1) Function and Localization

• Condensin complex subunit 1 (CNAP1), also known as CAP-D2, hCAP-D2, or XCAP-D2 homolog, is the regulatory subunit of the condensin I complex, encoded by the NCAPD2 gene in humans.
• It is essential for mitotic chromosome condensation, converting interphase chromatin into compact mitotic chromosomes by introducing positive supercoils into DNA in the presence of topoisomerases. Related references: PMID:11136719
• CNAP1 may target the condensin complex to DNA via its C-terminal domain, promoting resolution of DNA catenanes between sister chromatids and facilitating chromatid decatenation during anaphase.
• The protein localizes to the nucleus and cytoplasm, and associates with chromosomes during mitosis.
• Early in neurogenesis, CNAP1 plays a critical role in accurate mitotic chromosome condensation in neural stem cells, influencing neuron pool size and cortical development; mutations are associated with primary microcephaly. Related references: PMID:27737959
• Post-translational modifications include phosphorylation, and the protein is subject to proteomics identification.
• Key associated keywords: Cell cycle, Cell division, Chromosome, DNA condensation, Mitosis.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody detects a band at approximately 157 kDa; optimize blocking and antibody dilution according to standard protocols.
• The immunogen corresponds to amino acids 100-200 of human CNAP1, a conserved region; cross-reactivity with mouse is reported, but validation in the relevant sample system is recommended.
• For ELISA, consider direct or sandwich formats and validate the antibody's performance with appropriate positive and negative controls.
• Ensure samples are properly prepared to avoid degradation, as the large target protein may be sensitive to proteolysis.

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CamelBio provides diagnostic manufacturers and research institutions with streamlined access to critical IVD raw materials, including validated antibodies like this CNAP1 monoclonal antibody for mitosis and chromosome condensation studies. Our comprehensive portfolio covers antibody pairs, bulk ancillary reagents, and rare-target sourcing, supporting your workflow from concept to clinic.