CMPK1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P30085

Core Product Specifications and Parameters

Biological Background: CMPK1 Function and Localization

• CMPK1 encodes UMP-CMP kinase, also known as deoxycytidylate kinase (dCMP kinase), which plays a central role in pyrimidine nucleotide metabolism by catalyzing the ATP-dependent phosphorylation of UMP and CMP to UDP and CDP, respectively.
• The enzyme exhibits broad nucleoside diphosphate kinase activity, contributing to the maintenance of cellular nucleotide pools.
• CMPK1 is a member of the adenylate kinase family and is involved in de novo pyrimidine biosynthesis.
• The protein localizes to both the nucleus and cytoplasm, consistent with its role in nucleotide synthesis across cellular compartments.
• Post-translational modifications include acetylation and ubiquitination; it is also reported as a phosphoprotein, suggesting regulatory mechanisms.
• It is ubiquitously expressed across human tissues, reflecting its fundamental role in nucleotide metabolism.
• Alternative initiation and splicing produce multiple isoforms, potentially fine-tuning kinase activity in different contexts.

Experimental Guidance and Technical Tips

• Consider using the recommended WB, IF/ICC, IP, and ELISA protocols, but optimize antibody dilution and detection conditions according to your specific sample type and assay requirements.
• The immunogen encompasses the central kinase domain (aa 69-228), which is highly conserved; verify cross-reactivity with mouse and other species as indicated.
• For WB, a protein band at ~22 kDa is expected; take into account potential post-translational modifications that may shift the apparent molecular weight.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of high-quality IVD raw materials, including validated antibodies like this CMPK1 polyclonal antibody, optimized for diagnostic assay development and research applications. Our sourcing capabilities extend to monoclonal antibody pairs, bulk ancillary reagents, and custom raw-material identification for rare targets, supporting your projects from concept to clinic. Let us be your reliable partner in nucleotide metabolism and cancer biomarker research.