CLEC2B Rabbit pAb - Q92478

Core Product Specifications and Parameters

Biological Background: CLEC2B Function and Localization

• CLEC2B, also designated Activation-induced C-type lectin (AICL), is a member of the C-type lectin superfamily and is known by alternative names CLECSF2 and IFNRG1.
• It acts as a membrane-bound ligand that specifically engages the activating receptor NKp80 (KLRF1) on natural killer (NK) cells, thereby stimulating NK-cell cytotoxicity and cytokine production.
• Physiologically, this interaction triggers the cytolysis of malignant myeloid cells that express CLEC2B, highlighting a role in immune surveillance.
• CLEC2B is predominantly expressed in lymphoid tissues and is found in most hematopoietic cell types.
• Subcellularly, the protein localizes to the cell membrane and also resides in the Golgi apparatus membrane.
• As a glycoprotein, CLEC2B features a single transmembrane helix with a signal-anchor, and its structure is stabilized by disulfide bonds.
• Post-translational modifications include ubiquitination (Ubl conjugation), though its functional significance remains to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 40–120) targets the N-terminal extracellular region, which may confer specificity; validating the antibody in cell lines expressing endogenous CLEC2B is recommended.
• For Western blot (WB) applications, a band at approximately 17 kDa is expected; consider using reducing conditions and a suitable myeloid cell line as a positive control.
• Given the cross-reactivity with rat, the antibody may be applicable in rodent models; however, species-specific validation is advised.
• For ELISA, optimize coating conditions with recombinant CLEC2B protein and verify the dynamic range using a known standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This CLEC2B Rabbit pAb supports immune-oncology and NK cell research, and we can further assist with rare-target material procurement to accelerate diagnostic development.