Cleaved PARP1 p25 Rabbit Polyclonal Antibody for WB and ELISA - P09874

Core Product Specifications and Parameters

Biological Background: Cleaved PARP1 p25 Function and Localization

• PARP1 (Poly [ADP-ribose] polymerase 1) is a nuclear enzyme that mediates poly-ADP-ribosylation (PARylation) of target proteins, playing a central role in DNA damage repair by recognizing strand breaks and recruiting repair machinery. Related references: PMID:17177976, PMID:18055453, PMID:18172500
• Upon caspase-3/7 cleavage during apoptosis, PARP1 is cleaved into an N-terminal 25 kDa (p25) fragment and a C-terminal 89 kDa fragment; the p25 fragment promotes AIFM1-mediated apoptosis and irreversibly binds to DNA breaks, interfering with DNA repair and enhancing cell death. Related references: PMID:33168626
• PARP1 is primarily localized in the nucleus, nucleolus, and chromosomes, but can translocate to the cytoplasm during apoptosis; it contains multiple zinc-finger domains for DNA binding and an auto-poly-ADP-ribosylation domain.
• As a key DNA repair enzyme, PARP1 catalyzes serine, glutamate, and other amino acid ADP-ribosylations on histones and repair proteins, facilitating chromatin relaxation and recruitment of repair factors. Related references: PMID:19764761, PMID:25043379, PMID:28190768, PMID:33186521, PMID:34874266
• PARP1 also functions in transcription regulation, acting as both a repressor (by modulating chromatin structure) and a positive/negative regulator of transcription elongation, and is involved in replication fork progression. Related references: PMID:15607977, PMID:22464733, PMID:27256882, PMID:35393539, PMID:33412112
• PARP1 is implicated in innate immunity by negatively regulating the cGAS-STING pathway through cytosolic PARylation of cGAS. Related references: PMID:32315358, PMID:32844745, PMID:35460603
• Post-translational modifications of PARP1 include acetylation, phosphorylation, ubiquitination, and extensive auto-poly-ADP-ribosylation, which regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to the N-terminal region (amino acids 201–300) of the human cleaved PARP1 p25 fragment. In WB, ensure sample preparation under apoptotic conditions to generate the p25 cleavage product; consider using positive controls such as staurosporine-treated cells.
• For ELISA, this antibody may be used as a capture or detection antibody to specifically measure cleaved PARP1 levels; validate detection sensitivity and specificity with recombinant p25 protein or peptide competition assays.
• Cross-reactivity is reported for human; test in other species if needed, as sequence homology may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for DNA repair and apoptosis research. Whether you are developing diagnostic assays targeting PARP1 cleavage products or require rare-target raw-material sourcing, our team supports every stage from concept to clinic. Contact us for tailored solutions in biomarker detection and therapeutic monitoring.