Cleaved PARP1 p25 Rabbit mAb for WB, IP, ELISA - P09874

Core Product Specifications and Parameters

Biological Background: Cleaved PARP1 p25 Function and Localization

• Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair, including base excision repair and double-strand breaks. Related references: PMID:17177976 PMID:18055453 PMID:18172500
• Cleaved by caspase-3 and caspase-7 at a conserved site to generate the p25 fragment, which translocates to the cytoplasm and promotes AIFM1-mediated apoptosis. Related references: PMID:33168626
• Under normal conditions, localizes to the nucleus, nucleolus, and chromosomes; following cleavage, the p25 fragment localizes to the cytoplasm.
• Acts as a transcription regulator: binds to nucleosomes and modulates chromatin structure to repress RNA polymerase II, and both positively and negatively regulates transcription elongation. Related references: PMID:15607977 PMID:22464733
• Functions as a negative regulator of the cGAS-STING innate immunity pathway by mediating poly-ADP-ribosylation and inactivation of CGAS. Related references: PMID:32315358 PMID:32844745 PMID:35460603
• Following DNA damage, interacts with HPF1 to switch its amino acid specificity to serine, leading to histone ADP-ribosylation and chromatin decompaction. Related references: PMID:28190768 PMID:29954836 PMID:32028527

Experimental Guidance and Technical Tips

• For Western blot detection of cleaved p25, consider using nuclear extracts from apoptotic cells induced by etoposide or staurosporine.
• Validate antibody specificity by including caspase inhibitor–treated controls or PARP1 knockout lysates to confirm cleavage-band identity.
• In immunoprecipitation, optimize lysis buffer to preserve transient protein interactions, as PARP1 participates in dynamic repair complexes.
• For ELISA, pairing with a capture antibody against the N-terminal region may improve sensitivity for cleaved PARP1 detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For PARP1 and apoptosis-related research, we supply high-quality reagents to support diagnostic assay development from concept to clinic. Contact us for customized procurement solutions and technical consultation.