Cleaved PARP (Asp214) Rabbit pAb - Q921K2

Core Product Specifications and Parameters

Biological Background: Poly [ADP-ribose] polymerase Function and Localization

• Key DNA repair enzyme: PARP1 mediates poly-ADP-ribosylation (PARylation) of target proteins in response to DNA damage, recruiting repair machinery to single-strand breaks.
• Apoptosis marker: During apoptosis, caspase-3 and caspase-7 cleave full-length PARP1 (113 kDa) at Asp214, generating an 89 kDa fragment that irreversibly binds DNA breaks and blocks repair, promoting programmed cell death.
• AIFM1-mediated apoptosis: The cleaved fragment undergoes auto-PARylation and acts as a poly-ADP-ribose carrier, triggering AIFM1 translocation from mitochondria to the nucleus to induce large-scale DNA fragmentation.
• Subcellular distribution: Localizes primarily to the nucleus (nucleoplasm and nucleolus) and associates with chromatin; following cleavage, the fragment translocates to the cytosol.
• Functional domains: Contains zinc-finger motifs for DNA binding, an automodification domain, and a C-terminal catalytic domain that uses NAD+ as substrate.
• Gene family: Belongs to the PARP superfamily; Parp1 (Adprt1) is the founding member. It is also known as ADPRT, ARTD1, and PPOL.
• Post-translational modifications: Extensive auto-poly-ADP-ribosylation modulates its own activity and serves as a scaffold for other repair factors. Cleavage by caspases is a key regulatory event.
• Keywords: ADP-ribosylation, allosteric enzyme, apoptosis, chromosome, cytoplasm, DNA damage, DNA repair, DNA-binding, glycosyltransferase, immunity, innate immunity, isopeptide bond, metal-binding, NAD, nucleotidyltransferase, nucleus, proteomics identification, repeat, transcription, transcription regulation, transferase, zinc, zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen spans residues 201-300 of mouse PARP1, which includes the caspase-3/7 cleavage site at Asp214. This design enables specific recognition of the cleaved form in apoptosis experiments.
• For Western blotting (WB), use lysates from mouse cells or tissues treated with apoptosis inducers (e.g., staurosporine, etoposide) to enhance signal. The full-length protein runs at ~113 kDa, while the cleaved fragment appears at ~89 kDa.
• For ELISA, consider using the antibody as a capture or detection reagent in sandwich assays; pairing with a pan-PARP antibody allows differentiation of cleaved vs. total PARP.
• Validate antibody specificity using PARP1 knockout cell lines or caspase inhibitor controls to confirm the detected band disappears under non-apoptotic conditions.
• Avoid repeated freeze-thaw cycles to maintain antibody integrity; short-term storage at 4°C is acceptable, but long-term storage is recommended at -20°C or below.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Cleaved PARP (Asp214) Rabbit pAb suits apoptosis and DNA damage research applications. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets to support your assay development and manufacturing needs.