Cleaved IL-18 (Tyr37) Rabbit mAb for WB - Q14116

Core Product Specifications and Parameters

Biological Background: Cleaved IL-18 (Tyr37) Function and Localization

• Interleukin-18 (IL-18) is a pro-inflammatory cytokine originally named interferon gamma-inducing factor (IGIF) and also known as IL-1 gamma.
• Binds to IL18R1 and IL18RAP to form a ternary complex that activates NF-kappa-B, driving inflammatory mediator synthesis (PubMed:14528293, PubMed:25500532, PubMed:37993714). Related references: PMID:14528293, PMID:25500532, PMID:37993714
• Synergizes with IL-12 to potently induce interferon-gamma (IFNG) production from Th1 and NK cells, promoting cell-mediated immunity (PubMed:10653850). Related references: PMID:10653850
• Mature IL-18 is released extracellularly through the gasdermin-D (GSDMD) pore after pyroptosis, linking inflammation to programmed cell death (PubMed:33883744). Related references: PMID:33883744
• Located in the cytoplasm and cytosol, and secreted upon activation.
• Expressed in ovarian carcinoma tissues but not in normal ovarian epithelial cells; the ovarian tumor form exhibits resistance to caspase-1 and -4 processing.
• Keywords: Alternative splicing, Cytokine, Inflammatory response, Secreted.

Experimental Guidance and Technical Tips

• This antibody targets an epitope surrounding Tyr37 of human IL-18, which corresponds to a cleavage site that generates the mature, bioactive form. Ensure sample preparation preserves the cleaved state.
• For Western blot analysis, a specific band at approximately 22 kDa is expected; include appropriate molecular weight markers and loading controls.
• As IL-18 is secreted, consider analyzing both cell lysates and conditioned medium when studying release mechanisms.
• Cross-reactivity has been reported for human; for analysis in other species, evaluate sequence homology before testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by supplying validated monoclonal antibodies like this anti-cleaved IL-18 (Tyr37) clone, alongside optimized antibody pairs, bulk ancillary reagents, and custom sourcing for inflammatory cytokine panels. Whether scaling up a cytokine assay or procuring rare-target raw materials, our integrated supply and technical support bridge research to diagnostic application.