Cleaved Gasdermin D (C Terminal) Rabbit mAb - Q9D8T2

Core Product Specifications and Parameters

Biological Background: Gasdermin-D Function and Localization

• Gasdermin-D (Gsdmd) is the precursor of a pore-forming protein crucial for pyroptosis, a lytic pro-inflammatory cell death. Upon cleavage by inflammatory caspases (CASP1, CASP4/CASP11) after inflammasome activation, the N-terminal fragment oligomerizes into membrane pores of 10–15 nm diameter, allowing IL-1β and IL-18 release. Related references: PMID:26375003, PMID:26375259, PMID:26611636, PMID:27383986, PMID:29195811, PMID:29274245
• The gasdermin-D pore can also form on mitochondrial membranes, releasing mitochondrial DNA into the cytosol, and can directly kill extracellular bacteria by binding to bacterial cardiolipin and phosphoinositides. Related references: PMID:27383986, PMID:37001519
• Beyond pyroptosis, gasdermin-D has a transcription coactivator function: in the upper small intestine, CASP3/CASP7 cleavage generates an N-terminal fragment that translocates to the nucleus, serving as a coactivator for STAT1 to induce CIITA and MHC class II, thereby maintaining food tolerance. Related references: PMID:37327784
• Subcellular localization is dynamic: gasdermin-D is found in the cytoplasm, inflammasome complexes, plasma membrane, mitochondrion membrane, nucleus, and is secreted extracellularly after pore formation.
• Post-translational modifications include palmitoylation, phosphorylation, ubiquitination, and glycosylation; the protein also contains isopeptide bonds and transmembrane β-strands.
• Tissue specificity: highly expressed in mouse brain endothelial cells, linking gasdermin-D to blood-brain barrier integrity and neuroinflammatory processes.
• Allergen-induced cleavage: papain allergen can cleave gasdermin-D to form N-terminal pores that specifically release interleukin-33 (IL-33), driving type 2 immune responses. Related references: PMID:35749514, PMID:35794369

Experimental Guidance and Technical Tips

• Due to the immunogen design (C-terminal residues 277–376), this antibody may detect both full-length gasdermin-D (~53 kDa) and the C-terminal cleavage fragment (~22 kDa) in Western blot. Validate cleavage patterns using LPS/ATP-stimulated mouse macrophages or caspase-1/11 inhibitors.
• For immunoprecipitation, consider using the antibody to enrich full-length gasdermin-D or its C-terminal fragment; optimize lysis and washing conditions to preserve protein interactions.
• In ELISA applications, pairing this antibody with an N-terminal-specific detection antibody could enable discrimination between full-length and cleaved gasdermin-D.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents. This anti-Gasdermin D antibody supports studies in inflammasome biology and cell death, and CamelBio can source additional rare-target antibodies, recombinant proteins, and custom assay development components for your pyroptosis research panel.