Cleaved Gasdermin B Rabbit Polyclonal Antibody for WB and ELISA - Q8TAX9-6

Core Product Specifications and Parameters

Biological Background: Gasdermin B Function and Localization

• Gasdermin-B (GSDMB), also known as gasdermin-like protein, is a precursor of a pore-forming protein that mediates pyroptosis following cleavage by granzyme A (GZMA). Related references: PMID:36899106 PMID:36991122 PMID:36991125
• Upon activation, the N-terminal fragment translocates to the plasma membrane, binds inner leaflet lipids, and homooligomerizes to form pores of 10-15 nm inner diameter, triggering cell lysis.
• This process is a key mechanism in granzyme-mediated cell death and immune defense against intracellular pathogens.
• Isoform 6 of GSDMB, targeted by this antibody, has a calculated molecular weight of approximately 46.5 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200-300 of human Cleaved Gasdermin B, a region that may be exposed following proteolytic cleavage. Validate detection of both full-length and cleaved forms in relevant pyroptosis models.
• For WB, use appropriate protease inhibitors during sample preparation to preserve the labile cleaved fragment. Optimal dilution should be empirically determined.
• For ELISA, the polyclonal nature may enhance capture or detection sensitivity; consider pairing with a matched monoclonal for sandwich ELISA if quantitation is required.
• Since the antibody is raised in rabbit, avoid using rabbit-derived secondary detection systems in multiplex applications to prevent cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our anti-Gasdermin B antibody supports investigations into pyroptosis and cell death pathways, and we can assist in sourcing rare-target raw materials for your unique assay development needs. Whether you require scale-up support or specialized immunoreagents, CamelBio is your reliable partner from concept to clinic.