Cleaved Gasdermin B (C Terminal) Rabbit Monoclonal Antibody for WB and ELISA - Q8TAX9

Core Product Specifications and Parameters

Biological Background: Gasdermin-B (GSDMB) Function and Localization

• Gasdermin-B (GSDMB) is a member of the gasdermin family, functioning as a precursor of a pore-forming protein that mediates pyroptosis upon cleavage by granzyme A (GZMA) from cytotoxic T and NK cells (PubMed:32299851, PubMed:27281216).
  Related references:
  PMID:32299851
  PMID:27281216
• Upon cleavage, the N-terminal fragment (Gasdermin-B N-terminal) translocates to the plasma membrane, oligomerizes, and forms pores of 10-15 nm inner diameter, leading to cell lysis and pyroptosis (PubMed:32299851, PubMed:36599845, PubMed:36991122). The C-terminal fragment remains autoinhibitory in the full-length protein.
  Related references:
  PMID:32299851
  PMID:36599845
  PMID:36991122
• The gene GSDMB produces multiple isoforms through alternative splicing, which exhibit distinct lipid-binding specificities; some isoforms target eukaryotic cell membranes (e.g., binding phosphatidylinositol phosphates) while others preferentially bind bacterial lipids like lipid A and cardiolipin to mediate antibacterial pyroptosis (PubMed:34022140, PubMed:36157507).
  Related references:
  PMID:34022140
  PMID:36157507
• Gasdermin-B also has non-pyroptotic roles in epithelial maintenance and repair, promoting cell proliferation and migration via regulation of PTK2/FAK-mediated PDGFA phosphorylation (PubMed:35021065).
  Related references:
  PMID:35021065
• Subcellular localization includes the cytoplasm and cell membrane; the protein translocates to the membrane upon activation.
• Tissue expression is noted in proliferating cells of the gastrointestinal tract, including the basal layer of esophagus and stomach isthmus/neck, suggesting roles in epithelial homeostasis.
• Post-translational modifications include palmitoylation and ubiquitination; the protein is a lipoprotein with coiled-coil domains and a transmembrane beta strand region.

Experimental Guidance and Technical Tips

• The immunogen for this rabbit monoclonal antibody is a synthetic peptide surrounding Ser245 of human Gasdermin B (isoform NP_001159430.1). In Western blot, consider detecting the cleaved C-terminal fragment at approximately 18 kDa, in addition to full-length or isoform bands ranging from 45 to 47 kDa. Validate specificity using lysates from cells treated with granzyme A to induce cleavage.
• For ELISA applications, pair this antibody with appropriate capture/detection antibodies or use it for direct detection of Gasdermin B fragments. Optimize conditions and validate with recombinant protein or cell lysates from relevant human samples.
• Since the function of Gasdermin B involves membrane translocation and pore formation, fractionation assays or immunofluorescence could be considered to examine its subcellular redistribution upon activation, though such applications are not explicitly listed and require independent validation.
• As a rabbit monoclonal antibody, it offers high reproducibility and low lot-to-lot variation, making it suitable for long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides researchers and diagnostic manufacturers with reliable access to a comprehensive portfolio of IVD raw materials, including antibodies, antigens, and ancillary reagents. This Cleaved Gasdermin B (C Terminal) rabbit monoclonal antibody supports research into pyroptosis, cell death, and epithelial repair mechanisms. Beyond individual antibodies, CamelBio can supply validated antibody pairs, bulk reagents, and custom sourcing for rare target raw materials, streamlining your assay development from concept to clinic.