CLDN18 Rabbit pAb - P56856

Core Product Specifications and Parameters

Biological Background: Claudin-18 Function and Localization

• Claudin-18 is a tight junction protein encoded by the CLDN18 gene, with a molecular weight of approximately 28 kDa. It is a transmembrane protein involved in the formation and regulation of paracellular barriers.
• In the lung, Claudin-18 is essential for alveolar fluid homeostasis, regulating alveolar epithelial tight junction composition and ion transport, and is required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier.
• Claudin-18 acts to maintain epithelial progenitor cell proliferation and organ size by regulating the subcellular localization of YAP1, restricting its nuclear accumulation and downstream target gene transcription.
• It functions as a negative regulator of RANKL-induced osteoclast differentiation, potentially via TJP2/ZO-2 relocation, and mediates the osteoprotective effects of estrogen, acting independently of RANKL signaling pathways.
• In the alveolar microenvironment, Claudin-18 contributes to homeostasis by regulating pH, which limits T-cell activation and thereby helps restrict infection by pathogens such as C. neoformans.
• Claudin-18 is required for the formation of the gastric paracellular barrier, enabling the stomach to respond appropriately to gastric acidification.
• Subcellularly, Claudin-18 localizes to cell junctions, particularly tight junctions, and is found at lateral cell membranes.
• Tissue expression of Claudin-18 is restricted to the lung and stomach mucosa, where it is predominantly observed in epithelial cells of the pit region and gastric glands.
• The protein undergoes alternative splicing and phosphorylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain (amino acids 200–261) of human CLDN18. For western blotting (WB), consider using lung or stomach tissue lysates or cell lines expressing Claudin-18, as the predicted band size is ~28 kDa.
• Cross-reactivity with mouse and rat is confirmed, allowing use across commonly studied model organisms. Validate the antibody’s specificity in your specific sample matrix to ensure reliable detection.
• For ELISA, the antibody can be used to detect recombinant or native Claudin-18. Optimize coating and detection conditions based on your assay format.
• As with any polyclonal antibody, lot-to-lot variability may occur. We recommend including appropriate positive and negative controls and titrating the antibody for your application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering a full spectrum of validated antibodies, assay development reagents, and supporting services to accelerate your diagnostic pipeline. For researchers and manufacturers focusing on tight junction biology and gastrointestinal or pulmonary applications, our portfolio includes Claudin-18 antibodies and related products that can be seamlessly integrated into your workflows. Whether you need bulk quantities, rare target sourcing, or customized technical consultation, CamelBio is equipped to bridge your sourcing needs from concept to clinic.