Claudin18.2 Rabbit Monoclonal Antibody for IHC-P, ELISA - P56856

Core Product Specifications and Parameters

Biological Background: Claudin18.2 Function and Localization

• Claudin-18 is a tight junction protein belonging to the claudin family, encoded by the CLDN18 gene. Alternative splicing produces two major isoforms: Claudin18.1 (lung-specific) and Claudin18.2 (stomach-specific). This antibody targets the Claudin18.2 isoform.
• Claudin18.2 is essential for the formation of the gastric paracellular barrier, regulating ion and solute permeability across the mucosal epithelium. It is critical for the response to gastric acidification and maintenance of mucosal integrity.
• In the stomach, Claudin18.2 is predominantly expressed in epithelial cells of the pit region and base of gastric glands, including both exocrine and endocrine cells (at protein level).
• Claudin18.2 promotes epithelial progenitor cell proliferation and organ size regulation by controlling YAP1 localization. It restricts YAP1 nuclear entry and transcriptional activity, thus influencing tissue homeostasis.
• Claudin18.2 acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 from the nucleus. It also mediates osteoprotective effects of estrogen independently of RANKL signaling.
• The protein is localized to tight junctions at cell-cell contacts, specifically at lateral cell membranes. It contains four transmembrane helices and is a phosphoprotein, suggesting regulation by phosphorylation.
• Clinically, Claudin18.2 is a well-established biomarker and therapeutic target in gastric cancer, often retained or overexpressed in gastric adenocarcinomas, making it valuable for diagnostic antibody development.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (amino acids 1‑100) of human CLDN18.2, so this monoclonal antibody is expected to recognize the Claudin18.2 isoform. Verify specificity by comparing staining in stomach (Claudin18.2‑positive) and lung (Claudin18.1‑positive, Claudin18.2‑negative) tissues.
• For IHC‑P on formalin‑fixed paraffin‑embedded samples, consider heat‑induced epitope retrieval with citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) to unmask the epitope. Optimize antibody dilution and incubation conditions for your specific tissue and fixation protocol.
• In ELISA, this antibody may be used as a capture or detection reagent when paired with a suitable matched pair. Validate performance using recombinant Claudin18.2 protein or cell lysates from target‑expressing cells.
• Given the restricted expression of Claudin18.2 to gastric tissues, always include a positive control (e.g., gastric adenocarcinoma cell lines or stomach tissue sections) and a negative control to ensure staining specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one‑stop supplier of IVD raw materials, connecting diagnostic manufacturers, laboratories, and research institutes with high‑quality antibodies, antigen pairs, and ancillary reagents. This Claudin18.2 monoclonal antibody exemplifies our commitment to providing validated tools for gastric cancer research and diagnostic assay development. Beyond individual antibodies, we offer customized sourcing of rare targets, bulk reagent supply, and technical consulting to support your entire workflow from concept to clinic.