Citrulline-Histone H3 (R8 Citrullination) Rabbit Monoclonal Antibody (Q16695/P68431) for WB, DB, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, responsible for wrapping and compacting DNA into chromatin. This structural role is critical for regulating DNA accessibility and thereby controls fundamental processes such as transcription, DNA repair, replication, and chromosomal stability. Histones, including H3, are subject to a complex array of post-translational modifications—often termed the “histone code”—that dynamically modulate chromatin structure and gene expression.

This antibody specifically detects citrullination at arginine 8 (R8) of Histone H3. Citrullination, the conversion of arginine to citrulline, is mediated by peptidylarginine deiminases (PADs) and has been implicated in gene regulation, NETosis, and autoimmune diseases. Histone H3 is known to be citrullinated at multiple residues, including R3, R8, R17, and R26, and these modifications can influence chromatin compaction. Related references: PMID:16567635.

The two SWISS-PROT accessions covered by this antibody, Q16695 (Histone H3.1t) and P68431 (Histone H3.1), represent distinct histone variants. Q16695 is a testis-specific H3 variant expressed in testicular cells, whereas P68431 corresponds to the canonical replication-dependent Histone H3.1, which is ubiquitously expressed. Both localize to the nucleus and chromosome.

Histone H3 is extensively modified on its N-terminal tail, including acetylation, methylation, phosphorylation, citrullination, and many other marks. These modifications are dynamically regulated and serve as binding platforms for chromatin-modifying complexes, thereby governing cellular identity and response to environmental cues.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using 10–20 µg of whole-cell lysate per lane. Validate the antibody in the relevant sample system (e.g., stimulated cells with induced citrullination) and include appropriate positive and negative controls.
• For dot blot (DB) and ELISA, optimize the antigen coating concentration and buffer conditions to ensure specific detection of citrullinated Histone H3. A synthetic citrullinated peptide around R8 may serve as a positive control.
• Because citrullination can be transient and enzyme-dependent, sample preparation should preserve post-translational modifications; consider using PAD inhibitors or immediate lysis with appropriate phosphatase/protease inhibitors.
• This rabbit monoclonal antibody may be used to probe citrullination dynamics in various human sample types; cross-reactivity with other species is wide-range predicted, but end-users should verify in their specific experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, including specialized antibodies like this citrulline-Histone H3-R8 rabbit monoclonal antibody for epigenetic and citrullination research. Our one-stop platform offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing solutions for rare-target raw materials, supporting your IVD development from concept to clinic.