CITED2 Rabbit Polyclonal Antibody for WB and ELISA - Q99967

Core Product Specifications and Parameters

Biological Background: CITED2 Function and Localization

• CITED2 (Cbp/p300-interacting transactivator 2), also known as MRG-1 or P35srj, is a transcriptional coactivator that bridges TFAP2 transcription factors and the p300/CBP coactivator complex to stimulate TFAP2-mediated activation.
• It positively regulates TGF-beta signaling via the SMAD/p300/CBP complex and enhances the transcriptional activity of peroxisome proliferator-activated receptor alpha (PPARA) and estrogen receptors.
• CITED2 also acts as a transcriptional corepressor by interfering with the binding of HIF1A or STAT2 to the p300/CBP complex.
• In development, CITED2 participates in sex determination by stimulating SRY transcription, controls left-right patterning by potentiating NODAL-mediated transcription in the left lateral plate mesoderm, and is essential for adrenal cortex differentiation by upregulating NR5A1 via WT1.
• The protein associates with chromatin at the PITX2 P1 promoter region, indicating a direct role in gene regulation.
• It is localized to the nucleus and undergoes alternative splicing; it is implicated in stress responses and diseases such as atrial septal defect.
• Keywords: Activator, developmental protein, repressor, nucleus, stress response, transcription regulation.

Experimental Guidance and Technical Tips

• For Western blotting, test a dilution range (e.g., 1:500–1:2000) and use a nuclear extract or whole cell lysate from cells expressing CITED2 as a positive control. The predicted molecular weight is approximately 28 kDa; verify band size with molecular weight markers.
• For ELISA, immobilize the immunizing peptide or recombinant CITED2 protein and titrate the antibody to determine optimal working concentrations.
• Due to reported cross-reactivity with rat, this antibody may detect CITED2 in rat samples; validate performance in your specific experimental system.
• Optimize blocking and washing conditions to reduce non-specific binding typical of polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your sourcing partner for transcription regulation and developmental biology research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to streamline your workflow with reliable supply of CITED2 antibodies and related products.