CHRNA10 Rabbit pAb - Q9GZZ6

Core Product Specifications and Parameters

Biological Background: Neuronal Acetylcholine Receptor Subunit Alpha-10 Function and Localization

• CHRNA10 encodes the neuronal acetylcholine receptor subunit alpha-10, a ligand-gated ion channel component that assembles into pentameric nicotinic acetylcholine receptors (nAChRs).
• Heteromeric CHRNA9:CHRNA10 receptors exhibit high calcium permeability and mediate fast excitatory neurotransmission, particularly in the auditory system, where they are critical for synaptic transmission between efferent olivocochlear fibers and cochlear hair cells.
  Related references:
  PMID:11752216
• Activation of cochlear CHRNA9:CHRNA10 receptors leads to calcium influx and subsequent potassium channel opening, resulting in hair cell hyperpolarization and modulation of auditory sensitivity; this mechanism is implicated in protection against acoustic trauma.
  Related references:
  PMID:11752216
• CHRNA10 is expressed in inner ear, tonsil, immortalized B-cells, cultured T-cells, and peripheral blood lymphocytes, with additional roles suggested in keratinocyte adhesion (By similarity).
  Related references:
  PMID:11752216
  PMID:15531379
• The protein localizes to the synaptic cell membrane and plasma membrane, consistent with its function as a transmembrane ion channel.
• As a secretory pathway glycoprotein, CHRNA10 contains a signal peptide, disulfide bonds, and glycosylation sites, typical of nicotinic receptor subunits.

Experimental Guidance and Technical Tips

• Given the immunogen spans the extracellular domain (aa 25–240), this antibody is expected to detect the full‑length CHRNA10 protein (~50 kDa) in Western blot; additional bands may represent glycosylated or dimeric species.
• As a polyclonal antibody, lot‑to‑lot variability may occur; we recommend optimizing working concentrations and including a positive control (e.g., recombinant CHRNA10 protein) in each experiment.
• For ELISA, the recombinant immunogen can be used to validate antibody binding; indirect or sandwich ELISA conditions should be empirically tested.
• CHRNA10 is a multi‑pass transmembrane receptor; denaturing sample preparation with reducing agents and heat is advised for Western blotting.
• This antibody is validated on human samples; cross‑reactivity with other species is not guaranteed and should be verified by sequence alignment and experimental testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement pipeline for IVD raw materials, connecting diagnostic developers with high‑quality antibodies and recombinant proteins required for translational research and assay manufacturing. This CHRNA10 polyclonal antibody supports investigations into nAChR‑mediated auditory processing and related disorders, and complements our portfolios of validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials. For rare or challenging targets, our global sourcing network can accelerate your development from concept to clinic.