CHMP7 Rabbit pAb - Q8WUX9

Core Product Specifications and Parameters

Biological Background: CHMP7 Function and Localization

• Serves as an ESCRT-III-like protein required to recruit the ESCRT-III complex to the nuclear envelope during late anaphase. Related references: PMID:26040712

• Together with spastin (SPAST), facilitates nuclear envelope sealing and mitotic spindle disassembly at the end of mitosis. Related references: PMID:26040712 PMID:28242692

• Recruitment to the reforming nuclear envelope is mediated by the inner nuclear membrane protein LEMD2 during anaphase. Related references: PMID:28242692

• Participates in the endosomal sorting pathway, contributing to multivesicular body formation. Related references: PMID:16856878

• Localizes to both the cytoplasm and the nuclear envelope, with translocation dependent on cell cycle stage.

• Undergoes alternative splicing; contains a coiled-coil domain essential for its function; subject to phosphorylation.

• Also designated Chromatin-modifying protein 7, reflecting its role in nuclear envelope sealing and chromatin reorganization during mitosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 230–453, a region containing the coiled-coil domain; the antibody may detect full-length CHMP7 (~51 kDa) and potential isoforms.
• For WB, validate in human, mouse, and rat cell lysates as cross-reactivity is reported; a band near 51 kDa is expected.
• For ELISA, the recombinant immunogen fragment can serve as a positive control to establish assay sensitivity.
• Consider testing in synchronized cell populations to capture cell cycle-dependent localization changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibodies, bulk reagents, and customized services from concept to clinic. This anti-CHMP7 polyclonal antibody supports research into cell division and endosomal trafficking, with CamelBio providing reliable supply of this and related rare-target reagents. Our expertise streamlines your diagnostic development pipeline.