CGRP Rabbit mAb for WB, ELISA - P06881

Core Product Specifications and Parameters

Biological Background: Calcitonin gene-related peptide 1 Function and Localization

• Calcitonin gene-related peptide 1 (CGRP-I, CALCA) is a peptide hormone that induces potent vasodilation by binding to the CALCRL-RAMP1 receptor complex, leading to relaxation of vascular smooth muscle cells. Related references: PMID:1318039 PMID:33602864 PMID:9620797
• CGRP also elevates platelet cAMP levels, contributing to its vasodilatory and anti-thrombotic effects. Related references: PMID:1318039
• Beyond its cardiovascular role, CGRP is abundantly expressed in the central nervous system, where it acts as a neurotransmitter or neuromodulator. Related references: PMID:3492492
• CGRP1 can also bind and activate the calcitonin receptor-like receptor (CALCR) in complex with RAMP1 (AMYR1 receptor), expanding its functional repertoire. Related references: PMID:38603770
• The peptide is secreted extracellularly, consistent with its hormonal and paracrine signaling functions.
• CGRP is prominently expressed in the spinal cord, particularly in sensory neurons, mediating pain transmission and neurogenic inflammation.
• The mature CGRP peptide contains a disulfide bond and an amidated C-terminus, structural features essential for receptor binding and biological activity.
• The CALCA gene undergoes alternative splicing to produce both calcitonin and CGRP, with tissue-specific expression patterns.

Experimental Guidance and Technical Tips

• For Western blot, consider reducing and denaturing samples; CGRP is a small peptide (~15 kDa) and may require low molecular weight detection conditions.
• For ELISA, the antibody may be suitable for direct or indirect detection of human CGRP; validate with appropriate peptide standards.
• The immunogen spans the propeptide region (aa 25–125), potentially recognizing both precursor and mature forms; verify specificity in your experimental system.
• Due to the secreted nature of CGRP, consider analyzing cell culture supernatants or tissue extracts for optimal signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with validated antibodies and raw materials for cardiovascular and neuroscience research. For reliable detection of CGRP, we offer monoclonal antibodies optimized for WB and ELISA, backed by our comprehensive sourcing platform. Explore our catalog for bulk orders, custom services, and technical support.