CGA Rabbit Polyclonal Antibody for WB and ELISA - P01215

Core Product Specifications and Parameters

Biological Background: CGA Function and Localization

• CGA is the common alpha subunit of the heterodimeric glycoprotein hormones: thyroid-stimulating hormone (TSH), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (CG).
• It pairs with hormone-specific beta subunits to form bioactive hormones that bind receptors on target cells, activating downstream signaling pathways.
• The protein is secreted from anterior pituitary cells (for TSH, LH, and FSH) and placental trophoblasts (for CG).
• CGA contains multiple intramolecular disulfide bonds and is N‑glycosylated, which can influence its stability and immunoreactivity.
• The mature chain has a predicted molecular weight of approximately 13,075 Da (observed ~13 kDa on SDS-PAGE).
• Alternative names (e.g., GPHA1, FSHA, LHA, TSHA) reflect its shared role across the glycoprotein hormone family.
• In addition to its canonical endocrine functions, CGA may have localized signaling roles in reproductive tissues.

Experimental Guidance and Technical Tips

• This rabbit polyclonal was raised against a recombinant fragment (aa 25–116) of human CGA, which corresponds to most of the mature protein.
• For Western blot, reduce and denature samples to resolve the ~13 kDa band; use lysates from human pituitary, placenta, or hormone‑secreting cell lines.
• Due to glycosylation, the protein may migrate as a broader band or slightly higher apparent molecular weight; deglycosylation can be used to confirm specificity.
• In ELISA, coat plates with recombinant CGA or natural hormone preparations; the polyclonal can serve as a capture or detection antibody after appropriate pairing.
• Cross‑reactivity is annotated for human only; when working with other species, verify sequence homology of the immunogen region before use.

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