CES3 Rabbit pAb - Q6UWW8

Core Product Specifications and Parameters

Biological Background: Carboxylesterase 3 Function and Localization

• Carboxylesterase 3 (CES3), also known as liver carboxylesterase 31 homolog, is a serine esterase enzyme that plays a key role in the detoxification of xenobiotics and the metabolic activation of ester and amide prodrugs.
• CES3 localizes to the lumen of the endoplasmic reticulum, where it is part of the cellular machinery for drug metabolism and lipid homeostasis.
• It is predominantly expressed in the liver, colon, and small intestine, consistent with its role in first-pass metabolism of orally administered drugs.
• The enzyme catalyzes the hydrolysis of ester bonds with lower efficiency toward certain bulky substrates like CPT-11 (irinotecan), a camptothecin prodrug used in cancer therapy.
• CES3 undergoes co- and post-translational modifications including signal peptide cleavage, glycosylation, and disulfide bond formation, which are critical for its folding and enzymatic activity.
• The protein belongs to the carboxylesterase family and shares structural features with other mammalian carboxylesterases, including the catalytic triad and conserved disulfide bridges.
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity or regulation of expression in different tissues.
• As a reference proteome member with proteomics identification, CES3 serves as a reliable biomarker and target in pharmacokinetic and toxicological studies.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 269-568 of human CES3, a region likely conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blot, the predicted molecular weight is approximately 62 kDa; consider using appropriate positive controls such as liver or colon tissue lysates.
• In ELISA applications, recombinant CES3 or native protein from tissue extracts may serve as coating antigens; validate the assay with known standards.
• Given the tissue expression pattern, this antibody is well-suited for studying CES3 in hepatic, intestinal, and colonic samples.
• Since post-translational modifications like glycosylation can affect electrophoretic mobility, a slight band shift may be observed; treat samples with deglycosylation enzymes if band identity is uncertain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This CES3 polyclonal antibody is part of our extensive catalog supporting studies in drug metabolism and cancer prodrug activation. Beyond single reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare targets—empowering your IVD development from concept to clinic.