CENPE Rabbit Polyclonal Antibody for WB, ELISA - Q02224

Core Product Specifications and Parameters

Biological Background: CENPE Function and Localization

• CENPE is a microtubule plus-end-directed kinetochore motor protein essential for chromosome congression, the alignment of chromosomes at the metaphase plate. It drives lateral sliding of polar chromosomes along spindle microtubules towards the equator and aids in establishing stable kinetochore-microtubule attachments (PubMed:23891108, PubMed:25395579, PubMed:7889940). Related references: PMID:23891108 PMID:25395579 PMID:7889940
• The motor activity of CENPE is favored by detyrosinated microtubule tracks, facilitating the transport of pole-proximal chromosomes towards the spindle equator (PubMed:25908662). Related references: PMID:25908662
• CENPE acts as a processive bidirectional tracker of dynamic microtubule tips, enhancing kinetochore links with dynamic microtubule ends even after chromosome congression (PubMed:23955301). Related references: PMID:23955301
• Required for stable kinetochore-microtubule attachment formation and contributes positively to congression only when microtubules are stabilized; suppresses congression in NDC80-depleted cells (PubMed:25743205, PubMed:17535814). Related references: PMID:25743205 PMID:17535814
• Plays a critical role in spindle assembly checkpoint (SAC) activation by interacting with BUB1B, which activates its kinase activity, necessary for preventing aneuploidy due to single chromosome loss.
• Localizes to the kinetochore at centromeres, as well as to the cytoplasm and mitotic spindle during cell division.
• Post‑translational modifications include phosphorylation and methylation; prenylation and ubiquitination may also occur, as indicated by keywords.
• Mutations in CENPE are linked to primary microcephaly (MCPH13), highlighting its developmental importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal region (amino acids 2542–2701) of human CENPE. Although cross‑reactivity with mouse and rat is reported, validation in the relevant sample system is recommended.
• This antibody is suitable for Western blot (WB) and ELISA. For WB, consider the large molecular weight (~316 kDa) and use appropriate gel percentages and transfer conditions for high‑molecular‑weight proteins.
• For applications not listed (e.g., immunofluorescence), pilot experiments should be performed to determine optimal dilutions and conditions.

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CamelBio provides one‑stop access to IVD raw materials, including validated polyclonal antibodies like this CENPE antibody, to support your research in mitosis and cell cycle regulation. We supply bulk ancillary reagents and can assist in sourcing rare targets, ensuring your projects advance from concept to clinic with reliable materials.