CEND1 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q8N111

Core Product Specifications and Parameters

Biological Background: CEND1 Function and Localization

• CEND1 (Cell Cycle Exit and Neuronal Differentiation Protein 1), also known as BM88, is a neuron-specific membrane protein involved in neuronal differentiation.
• It is predicted to contain a transmembrane helix, anchoring it to cellular membranes.
• CEND1 is a phosphoprotein, suggesting regulation by phosphorylation signaling pathways.
• Its expression is restricted to neurons, highlighting a specialized role in the nervous system.
• The protein plays a role in promoting cell cycle exit and driving neuronal differentiation, though the exact mechanism is not fully detailed.
• UniProt keyword annotations indicate it is a reference proteome component and has been identified by proteomics.

Experimental Guidance and Technical Tips

• For Western blot, the predicted protein weight is ~15 kDa; consider using appropriate gel percentage and membrane transfer conditions for low molecular weight proteins.
• In immunofluorescence (IF-P), since CEND1 is a membrane protein, permeabilization may be required for optimal detection; validate in neuronal cell lines or tissue sections.
• The immunogen spans amino acids 70-149, which may recognize native and denatured forms; epitope accessibility might vary, so test different fixation/permeabilization protocols.
• For ELISA, the synthetic peptide immunogen suggests suitability for peptide-based assays; antibody performance in sandwich ELISA should be validated with recombinant antigen.
• Cross-reactivity is reported for human, mouse, and rat; check sequence homology in the immunogen region across species to anticipate reactivity in other organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated monoclonal antibodies like this CEND1 antibody for neuronal differentiation research. Our portfolio includes optimized antibody pairs, bulk ancillary reagents, and custom rare target sourcing to streamline your assay development from concept to clinic.