CEACAM7 Rabbit Polyclonal Antibody for WB and ELISA - Q14002

Core Product Specifications and Parameters

Biological Background: CEACAM7 Function and Localization

• CEACAM7 (Carcinoembryonic antigen-related cell adhesion molecule 7), also known as CGM2, is a member of the carcinoembryonic antigen (CEA) family of glycoproteins implicated in cell adhesion processes.
• The protein contains immunoglobulin-like domains and is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage, as indicated by keywords such as "Immunoglobulin domain", "Glycoprotein", and "GPI-anchor".
• CEACAM7 localizes to the cell membrane, specifically the apical cell membrane in polarized epithelial cells.
• Tissue expression is restricted to columnar epithelial cells of the colon, where it is strongly down-regulated in adenocarcinomas. Related references: PMID:10436421
• Structural features include a signal peptide, disulfide bonds, and multiple repeats, contributing to its role in cell-cell interactions.
• Alternative splicing generates multiple isoforms of CEACAM7, as noted in UniProt keywords.
• The protein has been identified through proteomics and direct protein sequencing, with its 3D structure resolved, underscoring its well-characterized molecular architecture.

Experimental Guidance and Technical Tips

• For Western blotting, consider using lysates from human colon tissue or relevant colorectal cell lines as CEACAM7 expression is predominant in colon epithelium. Validate band specificity with appropriate controls.
• For ELISA applications, the recombinant protein immunogen (aa 36-242) can serve as a positive control; optimize coating concentrations and detection antibody dilutions empirically.
• The antibody cross-reacts with mouse and rat CEACAM7, enabling cross-species studies; however, validate performance in the target species' sample system before use.
• Due to the GPI-anchored nature of CEACAM7, consider including mild detergents during sample preparation and membrane solubilization to ensure efficient extraction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For colorectal cancer and cell adhesion research targeting CEACAM7, we streamline access to reliable reagents backed by rigorous quality control. Our integrated supply chain supports diagnostic development from early concept through clinical application, helping you bridge the gap between discovery and commercial IVD products.