CDX2 Rabbit Polyclonal Antibody for IHC-P and ELISA - Q99626

Core Product Specifications and Parameters

Biological Background: CDX2 Function and Localization

• CDX2 is a homeobox transcription factor (homeobox protein CDX-2), also known as CDX-3 or caudal-type homeobox protein 2, encoded by the CDX2 gene in humans.
• It plays a critical role in early differentiation and maintenance of the intestinal epithelial lining of both the small and large intestine, regulating the transcription of multiple genes in the intestinal epithelium.
• CDX2 binds to specific DNA sequences in the promoter regions of target genes such as SI (sucrase-isomaltase), VDR, LPH, CLDN2, MUC2, and CA1, thereby activating their transcription.
• It binds preferentially to methylated DNA, suggesting a role in epigenetic regulation of gene expression. Related references: PMID:28473536
• CDX2 is localized to the nucleus, consistent with its function as a transcription factor.
• Expression is detected in the small intestine, colon, and pancreas, reflecting its tissue-specific regulatory roles.
• Important keywords associated with CDX2 include: activator, developmental protein, DNA-binding, homeobox, nucleus, phosphoprotein, reference proteome, transcription, transcription regulation, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (amino acids 1–180) of human CDX2; consider using this recombinant protein as a positive control in ELISA experiments.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), validate the antibody in relevant intestinal or colon tissue samples from human, mouse, or rat. Antigen retrieval optimization may be necessary.
• As a polyclonal antibody, lot-to-lot consistency should be monitored. Include appropriate negative controls (e.g., non-immune rabbit IgG) and confirm specificity through knockdown or knockout models where possible.
• The predicted molecular weight of CDX2 is ~34 kDa; use appropriate molecular weight markers for Western blot if adapting the antibody for this application, though it is validated only for IHC-P and ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored for diagnostic development. This CDX2 polyclonal antibody supports research on intestinal differentiation and related pathologies, and CamelBio can assist with sourcing rare-target reagents, custom antibody generation, and technical consulting to streamline your project from concept to clinic.