CDX1 Rabbit Polyclonal Antibody for WB, ELISA - P47902

Core Product Specifications and Parameters

Biological Background: CDX1 Function and Localization

• CDX1 is a homeobox protein that functions as a transcription factor.
• It contributes to transcriptional regulation in colorectal cancer, mediating activated KRAS-induced PRKD1 expression by promoter binding. Related references: PMID:24623306
• The protein is implicated in intestinal terminal differentiation.
• Preferentially binds to methylated DNA. Related references: PMID:28473536
• Subcellular localization is nuclear, aligning with its role in gene transcription.
• It is primarily expressed in the intestinal epithelium.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against a recombinant fragment of human CDX1 (aa136-265), is recommended for WB and ELISA.
• For WB, the expected protein molecular weight is approximately 28kDa; use intestinal or colorectal cancer cell lines as positive controls.
• For ELISA, determine the optimal coating antigen and antibody concentrations empirically.
• Cross-reactivity with mouse CDX1 has been reported, enabling potential use in mouse model studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop sourcing platform for IVD raw materials, covering every stage from concept to clinic. Our CDX1 polyclonal antibody serves as a reliable reagent for studying intestinal development and colorectal cancer transcriptional pathways. Complement your projects with our extensive portfolio of validated antibody pairs, bulk ancillary reagents, and specialized sourcing services for rare-target raw materials.