CDKN2A/p16INK4a Rabbit pAb for WB, ELISA - P42771 / Q8N726

Core Product Specifications and Parameters

Biological Background: CDKN2A/p16INK4a Function and Localization

• The CDKN2A gene encodes cyclin-dependent kinase inhibitor 2A, also known as p16-INK4a, CDK4I, and MTS-1, with multiple transcript variants arising from alternative splicing.
• It functions primarily as a negative regulator of cell proliferation by binding strongly to CDK4 and CDK6, thereby inhibiting their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
• Subcellular localization includes both the cytoplasm and the nucleus, facilitating its regulatory activities in cell cycle control.
• Tissue expression is widespread, though not detected in brain or skeletal muscle; a pancreas-specific isoform (isoform 3) has been identified.
• Post-translational modifications reported include acetylation and phosphorylation, which may modulate its stability and interactions.
• The protein contains ANK repeats and is classified as a tumor suppressor, with mutations associated with disease syndromes such as Li-Fraumeni.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length p16INK4a isoform (aa 1‑156); due to alternative splicing, the antibody may detect multiple isoforms. Validate in the relevant sample system.
• For Western blotting, consider the expected molecular weight range of 8 ‑ 17 kDa depending on the isoform expressed; include appropriate controls.
• ELISA may be used for quantitative detection; optimal antibody concentration and assay conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. The CDKN2A/p16INK4a antibody is suited for cell cycle, tumor biology, and cancer diagnostic developments, and CamelBio can support your entire reagent supply chain, from preclinical assessment to commercial scale-up.