CDK9 Rabbit Polyclonal Antibody for WB and ELISA - P50750

Core Product Specifications and Parameters

Biological Background: CDK9 Function and Localization

• CDK9 is a serine/threonine-protein kinase that forms the catalytic subunit of the positive transcription elongation factor b (P-TEFb) complex with cyclin T. This complex is essential for transcription elongation by RNA polymerase II. Related references: PMID:10574912, PMID:10757782, PMID:11145967

• P-TEFb phosphorylates the C-terminal domain of RNA polymerase II (POLR2A), as well as negative elongation factors DSIF and NELF, to relieve their inhibitory effects on transcription. It also phosphorylates transcription factors such as EP300, MYOD1, and AR. Related references: PMID:10912001, PMID:11112772, PMID:12037670

• CDK9 modulates a complex network of chromatin modifications, including histone H2B monoubiquitination (H2Bub1), H3K4 trimethylation (H3K4me3), and H3K36me3, integrating transcription with co-transcriptional histone mRNA processing. Related references: PMID:15564463, PMID:19575011, PMID:19844166

• CDK9 phosphorylates histone H1.4 at Ser-187, a mark associated with transcriptional activation. Related references: PMID:28539972

• The kinase plays a role in replication stress response and genome integrity maintenance through the CDK9/cyclin-K complex, promoting cell cycle recovery from replication arrest and limiting DNA damage. Related references: PMID:20493174

• Subcellularly, CDK9 is found in the nucleus, PML bodies, and cytoplasm, and is ubiquitously expressed in human tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 153-372 of human CDK9, a region conserved across human, mouse, and rat, supporting the reported cross-reactivity. Consider verifying performance in species-specific sample systems.
• For Western blot, the predicted molecular weight is 43 kDa. Use appropriate positive control lysates (e.g., HeLa, Jurkat) and validate band specificity with knockdown controls or blocking peptides.
• For ELISA, the antibody may detect both native and recombinant CDK9. Optimize coating antigen concentration and perform titration experiments to establish working dilutions.
• Consider the ubiquitous expression pattern of CDK9 when selecting negative controls and interpreting results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a comprehensive portfolio of validated antibodies, including this CDK9 polyclonal antibody for transcription and cell cycle research. Our integrated supply chain provides one-stop access to bulk ancillary reagents, optimized antibody pairs, and rare target raw materials, supporting your IVD development from concept to clinic.