CDK4 Rabbit Polyclonal Antibody [KO Validated] for WB, IHC-P, IF/ICC, IP, ELISA - P11802

Core Product Specifications and Parameters

Biological Background: CDK4 Function and Localization

• Cyclin-dependent kinase 4 (CDK4) is a Ser/Thr-protein kinase that forms active complexes with D-type cyclins, primarily regulating G1/S cell cycle transition.
• CDK4 phosphorylates the retinoblastoma protein (RB1), leading to the dissociation and activation of E2F transcription factors, which drive the expression of genes required for S-phase entry.
• It hypophosphorylates RB1 in early G1 and serves as a major integrator of mitogenic and anti-mitogenic signaling pathways.
• Additionally, CDK4 phosphorylates SMAD3 in a cell-cycle-dependent manner, repressing its transcriptional activity.
• Nuclear translocation and full activity of cyclin D-CDK4 require assembly into a ternary complex with CDKN1B (p27Kip1).
• Subcellular localization includes cytoplasm, nucleus, and nuclear membrane.
• Post-translational modifications include acetylation; alternative splicing produces distinct isoforms.
• CDK4 is implicated in various diseases through gain-of-function variants and is a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen was designed from a synthetic peptide spanning amino acids 204-303 of human CDK4. This region lies within the C-terminal domain and may contribute to antibody specificity.
• For Western blotting (WB), consider using whole-cell lysates from proliferating human, mouse, or rat cell lines. Endogenous CDK4 migrates at ~34 kDa; the antibody detects the full-length protein.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization steps may be required. Always validate the staining pattern in a relevant tissue or cell system.
• The antibody is suitable for immunoprecipitation (IP) and ELISA as indicated; users should optimize conditions and confirm results through parallel controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for cell cycle and oncology research. We support CDK4-targeted assay development with comprehensive supply solutions and technical consulting, bridging the gap from concept to clinic.