CDK4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P11802

Core Product Specifications and Parameters

Biological Background: CDK4 Function and Localization

• CDK4 is a serine/threonine kinase that forms active cyclin D-CDK4 complexes, which phosphorylate retinoblastoma (RB) family proteins, including RB1, regulating the G1/S cell cycle transition.
• Phosphorylation of RB1 by cyclin D-CDK4 leads to dissociation of E2F transcription factors, enabling transcription of genes required for S-phase entry.
• Cyclin D-CDK4 complexes integrate mitogenic and antimitogenic signals to control cell proliferation.
• CDK4 also phosphorylates SMAD3 in a cell-cycle-dependent manner, repressing its transcriptional activity.
• The protein localizes to the cytoplasm, nucleus, and nuclear membrane; nuclear translocation is facilitated by the cyclin D/CDK4/CDKN1B ternary complex.
• CDK4 undergoes acetylation and phosphorylation, and it is an ATP-binding kinase; alternative splicing generates multiple isoforms, and mutations are linked to various cancers.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (a.a. 200-303) of human CDK4, which is likely conserved, supporting the reported cross-reactivity with mouse and rat.
• For Western blotting, expect a band near 34 kDa; validate detection in proliferating cell lines and use appropriate loading controls.
• In immunohistochemistry, antigen retrieval conditions may be optimized; nuclear and/or cytoplasmic staining may be observed depending on the cell cycle stage.
• For ELISA applications, consider the antibody as a capture or detection reagent; pairing with other CDK4 antibodies may improve assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop shop for IVD raw materials, from validated monoclonal antibodies like this CDK4 Rabbit mAb to bulk ancillary reagents and custom rare-target sourcing. Whether you need optimized antibody pairs for sandwich immunoassays or support with cell cycle or oncology biomarker projects, CamelBio streamlines your development from concept to clinic.