CDK1/2 Rabbit PolymAb® Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P06493/P24941

Core Product Specifications and Parameters

Biological Background: CDK1/2 Function and Localization

• This antibody detects CDK1 and CDK2, two closely related cyclin-dependent kinases that are central regulators of the eukaryotic cell cycle.
• CDK1 plays a key role in driving the G2-M transition by phosphorylating numerous substrates required for mitotic entry and progression. Related references: PMID:16407259 PMID:16933150 PMID:17459720
• CDK1 catalyzes lamin (LMNA, LMNB1, LMNB2) phosphorylation at mitotic onset, leading to nuclear envelope breakdown. Related references: PMID:2188730 PMID:2344612 PMID:37788673
• Its activity is tightly controlled by phosphorylation: activated by the CDK-activating kinase (CDK7/cyclin H/MAT1) and CDC25 phosphatases, while inhibited by WEE1 and PKMYT1 kinases. Related references: PMID:18480403 PMID:20360007 PMID:2188730 PMID:41100585 PMID:20395957
• CDK1 phosphorylates beta-tubulins, NEDD1, and CC2D1A to regulate microtubule dynamics, centrosome maturation, and spindle assembly during mitosis. Related references: PMID:16371510 PMID:19509060 PMID:20171170
• In apoptosis, CDK1-mediated phosphorylation of Bcl-xL promotes cell death, whereas CASP8 phosphorylation inhibits its processing and apoptosis. Related references: PMID:19917720 PMID:20937773
• CDK1 contributes to epigenetic regulation by phosphorylating EZH2, maintaining H3K27me3 marks and gene silencing. Related references: PMID:20935635
• Subcellularly, CDK1 localizes to the nucleus, cytoplasm, mitochondria, centrosome, and mitotic spindle, in line with its diverse mitotic roles.

Experimental Guidance and Technical Tips

• For Western blotting, use proliferating cell lysates (e.g., HeLa, Jurkat) as positive controls; consider cell cycle synchronization to enhance CDK1/2 signals.
• In immunohistochemistry (IHC-P), validate antigen retrieval conditions and include tissues with known proliferative indices, such as breast cancer samples.
• For immunofluorescence (IF-P), co-stain with centriolar or mitotic markers (e.g., γ-tubulin, pericentrin) to confirm localization.
• The antibody’s immunogen (CDK2 aa 199-298) is conserved in CDK1; cross-reactivity is confirmed in human and mouse samples, but validation in other species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive selection of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This CDK1/2 PolymAb® supports cell cycle and oncology research, offering reliable detection across multiple applications. For rare targets or custom sourcing needs, CamelBio’s global network streamlines procurement from lead candidate identification to clinical assay development.