CDC25C Rabbit pAb for IF/ICC, ELISA - P30307

Core Product Specifications and Parameters

Biological Background: CDC25C Function and Localization

• CDC25C, also known as M-phase inducer phosphatase 3 or dual specificity phosphatase Cdc25C, is a member of the CDC25 phosphatase family that plays a critical role in cell cycle regulation.
• It functions as a dosage-dependent mitotic inducer, directly dephosphorylating and activating CDK1 to drive G2/M transition and mitotic entry.
  Related references: PMID:8119945
• As a dual specificity phosphatase, CDC25C removes inhibitory phosphate groups from both threonine and tyrosine residues on CDK1.
• The protein is primarily localized in the nucleus, consistent with its role in controlling nuclear events during mitosis.
• CDC25C is subject to regulatory post-translational modifications, including phosphorylation and acetylation, which modulate its activity and stability.
• Alternative splicing generates multiple isoforms, potentially adding functional diversity.
• It is also implicated in host-virus interactions, highlighting its broader cellular significance.
• Keywords associated with CDC25C include cell cycle, cell division, mitosis, hydrolase, and phosphoprotein, reflecting its enzymatic and regulatory roles.

Experimental Guidance and Technical Tips

• The polyclonal nature of this antibody may introduce batch-to-batch variability; consider validating each lot in the intended sample system.
• For IF/ICC, optimize fixation and permeabilization conditions, as nuclear staining of CDC25C may require specific protocols to preserve antigenicity.
• In ELISA, the antibody could be used as either a capture or detection reagent, but careful pairing and titration are recommended.
• The immunogen (amino acids 174-473) encompasses the catalytic domain, suggesting potential recognition of both native and denatured CDC25C; this can be leveraged in assays requiring conformational sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined sourcing platform for IVD raw materials, including validated antibodies like this anti-CDC25C polyclonal antibody for cell cycle and oncology applications. Beyond core products, we offer complementary ancillary reagents, bulk supply options, and customized support to accelerate your assay development. Leverage our integrated supply chain to secure all essential raw materials in one step.