CD79B Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P40259

Core Product Specifications and Parameters

Biological Background: CD79B Function and Localization

• CD79B (B-cell antigen receptor complex-associated protein beta chain) is also known as B29, Ig-beta, and immunoglobulin-associated B29 protein. Alternative splicing generates multiple isoforms, though the functional significance is context-dependent.
• Together with CD79A, CD79B is required for initiating the signal transduction cascade upon B-cell antigen receptor (BCR) engagement, leading to receptor internalization, trafficking to late endosomes, and subsequent antigen presentation.
• CD79B enhances phosphorylation of CD79A, potentially by recruiting kinases that phosphorylate CD79A or by attracting proteins that protect it from dephosphorylation, thus amplifying BCR signaling.
• Structurally, CD79B belongs to the immunoglobulin superfamily and contains an extracellular immunoglobulin domain, a single-pass transmembrane helix, and a cytoplasmic tail bearing an immunoreceptor tyrosine-based activation motif (ITAM).
• The protein localizes exclusively to the cell membrane, where it forms a disulfide-linked heterodimer with CD79A as part of the BCR complex.
• CD79B expression is restricted to B-cells, consistent with its role in adaptive immunity. It is often used as a pan-B-cell marker in diagnostic immunohistochemistry.
• Post-translational modifications include glycosylation, phosphorylation at key tyrosine residues within the ITAM, and ubiquitination. The protein’s three-dimensional structure has been solved, and it is classified under several UniProt keywords including adaptive immunity, membrane receptor, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–100 of human CD79B, encompassing the extracellular domain. This region is highly conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For western blotting, typical samples include whole‑cell lysates from human B‑cell lines (e.g., Raji, Daudi) or mouse/rat splenocytes; a band near 26 kDa is expected.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), membrane staining is anticipated in B‑cell areas of lymphoid tissues (e.g., lymph node, spleen, tonsil). Adequate antigen retrieval may be required for formalin‑fixed paraffin‑embedded sections.
• For ELISA applications, the antibody can be used as capture or detection reagent against recombinant CD79B or B‑cell lysates; validate performance in your specific assay matrix.
• As with any antibody, verify reactivity and optimal working dilutions in your experimental system, especially when using tissues from different species or pathological states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined procurement pathway for high-quality IVD raw materials, including this CD79A/B rabbit monoclonal antibody tailored for B‑cell receptor signaling studies and immunoassay development. Beyond individual antibodies, our portfolio extends to validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and rare-target sourcing—all supported by technical consultation from concept to clinic. Partner with CamelBio to accelerate your next diagnostic project with reliable, ready-to-scale raw materials.