CD79a Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, FC, ELISA - P11912

Core Product Specifications and Parameters

Biological Background: CD79a Function and Localization

• CD79a, also known as Ig-alpha or MB-1, is the alpha subunit of the B-cell antigen receptor complex. It contains an immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail.
• Together with CD79b, it forms the signaling component of the BCR. Antigen binding triggers intracellular signal transduction, leading to receptor internalization and antigen presentation.
• Essential for BCR surface expression and efficient differentiation of pro- and pre-B-cells.
• Functions in signal initiation: stimulates SYK autophosphorylation and activation, recruits BLNK for phosphorylation, and modulates Src-family tyrosine kinase activity.
• During immature B-cell development, CD79a also represses BCR signaling, contributing to tolerance checkpoints.
• Subcellularly localized to the cell membrane, with expression restricted to B-cells.
• Post-translational modifications include glycosylation, phosphorylation, methylation, and ubiquitination; stabilized by disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic tail (aa 165–225). Thus, the antibody likely detects an intracellular epitope; permeabilization is recommended for flow cytometry and immunofluorescence applications.
• For Western blotting, expect a specific band at ~25 kDa under reducing conditions. Validate the signal using established B-cell lines (e.g., Ramos, Raji).
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced, pH 6 or 9) may enhance staining. Look for membranous/cytoplasmic signals in B-cell zones of lymphoid tissues.
• Flow cytometry assays should include permeabilization steps; confirm CD79a expression in peripheral blood B-cells or B-lymphoblastoid cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to critical IVD raw materials, such as this CD79a monoclonal antibody, optimized for B-cell receptor studies and hematopathology applications. Our comprehensive supply network includes validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, ensuring seamless support from early concept to clinical deployment.