CD70 Rabbit pAb - P32970

Core Product Specifications and Parameters

Biological Background: CD70 Function and Localization

• CD70 is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) ligand superfamily and is also known as CD27 ligand (CD27L) or TNFSF7.
• It is primarily expressed on activated B cells, where it serves as the ligand for the CD27 receptor present on T cells, mediating the CD70-CD27 signaling pathway that is critical for antigen-specific T cell activation and expansion (PubMed:28011863, PubMed:28011864). Related references: PMID:28011863 PMID:28011864
• The CD70-CD27 interaction provides co-stimulatory signals that promote T cell proliferation, survival, and effector function, contributing to immune surveillance of B cells (PubMed:8387892). Related references: PMID:8387892
• CD70 is localized at the cell membrane with a single-pass transmembrane domain and an extracellular TNF homology domain that mediates receptor binding.
• Alternative splicing of the CD70 gene generates multiple transcript variants, which may influence protein expression and function.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for proper protein folding and stability.
• CD70 is classified under keywords including "Disease variant", "Glycoprotein", and "Signal-anchor", indicating its involvement in pathological conditions and its membrane anchoring mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 47-126 of human CD70, which encompasses the extracellular TNF domain; therefore, the antibody is expected to recognize the ectodomain of CD70.
• For Western blotting, consider using reducing conditions with DTT or β-mercaptoethanol to ensure complete denaturation of disulfide bond-stabilized structures, which may affect migration.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody in relevant positive control tissues (e.g., activated B-cell lymphomas or lymphoid tissues) to confirm membrane staining patterns.
• When performing ELISA, pair with a CD70-specific capture antibody or use direct coating of recombinant CD70 to establish a robust standard curve.
• Due to cross-reactivity with mouse and rat, verify sequence homology of the immunogen epitope in these species to ensure expected reactivity in your experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to a comprehensive portfolio of IVD raw materials. For CD70-related research—including immuno-oncology, immune checkpoint studies, and tumor microenvironment analysis—we offer optimized polyclonal and monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents. Our sourcing capabilities also extend to rare and custom targets, helping you accelerate development from concept to clinical application.