CD7 Rabbit pAb - P09564

Core Product Specifications and Parameters

Biological Background: CD7 Function and Localization

• CD7 is a transmembrane glycoprotein and member of the immunoglobulin superfamily, expressed primarily on T-cells and natural killer (NK) cells and their precursors. Related references: PMID:7506726
• Upon binding to its ligand K12/SECTM1, CD7 provides a costimulatory signal that enhances T-cell activation. Related references: PMID:10652336
• CD7 engagement promotes the production of cytokines such as IL-2, thereby modulating adaptive immune responses. Related references: PMID:1709867
• On resting NK cells, CD7 cross-linking triggers a significant increase in interferon-gamma (IFN-γ) production, highlighting its role in innate immunity. Related references: PMID:7506726
• CD7 localizes to the plasma membrane with a single-pass transmembrane domain.
• The protein contains an extracellular immunoglobulin-like domain, a disulfide bond, and is palmitoylated, suggesting lipid raft association.
• CD7 is a lineage-specific marker expressed on T-cells and NK cells and their precursors, absent on B-cells and myeloid cells.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the extracellular domain (aa 26-180), enabling recognition of surface-expressed CD7.
• For Western blot, expect a specific band around 25 kDa (theoretical molecular weight) in T-cell lysates; cross-reactivity with rat may require validation.
• In flow cytometry, this rabbit polyclonal antibody can detect native CD7 on human and rat T-cells; consider using a secondary anti-rabbit IgG conjugate.
• For ELISA, the antibody may be used as capture or detection reagent; optimal dilutions and conditions should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of validated antibodies and ancillary reagents for immunology and diagnostics research. Our CD7 Rabbit pAb supports studies on T-cell and NK cell activation, and we offer one-stop access to optimized antibody pairs, monoclonal/polyclonal antibodies, and bulk reagents. From concept to clinic, we also assist with rare target raw-material sourcing to accelerate your IVD development.