CD59 Rabbit pAb - P13987

Core Product Specifications and Parameters

Biological Background: CD59 Function and Localization

• CD59 glycoprotein, also known as protectin, HRF-20, MACIF, MIRL, and MEM43 antigen, is a potent inhibitor of the complement membrane attack complex (MAC).

Related references: PMID:11882685, PMID:1698710, PMID:2475111, PMID:36797260

• It acts by binding to the C8 and C9 components of the assembling MAC, preventing incorporation of multiple C9 molecules and thus blocking formation of the osmolytic pore.

Related references: PMID:11882685, PMID:1698710, PMID:36797260

• The soluble form of CD59 (found in urine) retains complement binding activity but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
• CD59 is predominantly localized on the cell membrane via a GPI anchor and is also secreted.
• The protein undergoes alternative splicing, and post-translational modifications include disulfide bond formation, glycation, and N-linked glycosylation.
• Mutations in the CD59 gene are associated with hereditary hemolytic anemia, reflecting its critical role in protecting cells from complement-mediated lysis.

Experimental Guidance and Technical Tips

• The immunogen (aa 26-128 of human CD59) corresponds to the extracellular domain, which is exposed on the cell surface and likely accessible in both native and denatured conditions, supporting WB, IHC-P, and ELISA applications.
• For IHC-P detection of this membrane-anchored antigen, consider antigen retrieval and validate in tissues known to express CD59, such as kidney, vascular endothelium, or hematopoietic cells.
• Because CD59 is a GPI-anchored protein, it may localize to lipid rafts; using appropriate lysis buffers or detergent-resistant membrane fractions may enhance detection in WB.
• The reported cross-reactivity with mouse and rat suggests a conserved epitope, but users should confirm reactivity in their species and sample type of interest.
• Include appropriate positive and negative controls (e.g., CD59-overexpressing lysates or CD59-silenced cells) to confirm antibody specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for complement system targets like CD59. We streamline the sourcing of rare-target raw materials and can tailor procurement to meet your assay development or large-scale production needs, bridging the gap from concept to clinic.