CD53 Polyclonal Antibody (Rabbit) for WB and ELISA - Q61451

Core Product Specifications and Parameters

Biological Background: CD53 Function and Localization

• CD53 (also known as Ox-44 or Tspan25) is a cell surface glycoprotein belonging to the tetraspanin family, characterized by four transmembrane domains.
• It serves as a structural component of tetraspanin-enriched microdomains (TERMs), which facilitate receptor clustering and signaling on the plasma membrane. Related references: PMID:36581203
• CD53 participates in diverse cellular processes, including signal transduction, adhesion, migration, and protein trafficking. Related references: PMID:32428859, PMID:32532837
• In B-cells, CD53 is essential for development by promoting interleukin-7 receptor (IL7R) signaling and enhancing BCR signaling through PKC recruitment to the membrane. Related references: PMID:31748347
• CD53 regulates lymphocyte homing to lymph nodes by stabilizing L-selectin (SELL) expression on the cell surface. Related references: PMID:32428859
• Beyond the immune system, CD53 mediates metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF and LPS signaling. Related references: PMID:36581203
• Under stress conditions, CD53 protects hematopoietic stem cell function by facilitating DREAM complex activity through interactions with p130/RBL2 and PP2A phosphatase. Related references: PMID:36542833
• Subcellular localization includes the cell membrane and cell junctions; the protein is extensively glycosylated and contains multiple transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 107-181 of mouse CD53, a region likely exposed on the extracellular side of the membrane; consider this when selecting detection or capture formats.
• For Western blotting, a band around 24 kDa is expected under reducing conditions; validate antibody specificity using positive controls such as mouse spleen or lymph node lysates.
• In ELISA, test the antibody as a capture or detection reagent, and optimize coating conditions and blocking to minimize background.
• As a polyclonal antibody, lot-to-lot consistency should be verified in your specific assay system; preliminary titration experiments are recommended.
• Although not listed, the antibody may also work in other applications like IP or IHC; however, users should empirically validate performance in their experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined path to procurement of high-quality raw materials. With expertise in targets like CD53—a key player in immunology and cell signaling—we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for sourcing rare-target raw materials. Partner with us to accelerate your assay development and manufacturing scale-up.