CD42b Rabbit mAb for WB, IHC-P, ELISA - P07359

Core Product Specifications and Parameters

Biological Background: CD42b Function and Localization

• CD42b is the alpha chain of the platelet glycoprotein Ib (GPIb) complex, a receptor for von Willebrand factor (vWF) on the platelet surface.
• It mediates initial platelet adhesion to the subendothelium by binding to the A1 domain of vWF, primarily under high shear stress in arteries.
• The protein contains several leucine-rich repeat (LRR) motifs, which facilitate protein-protein interactions and are essential for ligand binding.
• CD42b is critical for primary hemostasis, as it orchestrates platelet plug formation at sites of vascular injury.
• Mutations in the GP1BA gene can lead to bleeding disorders, including Bernard-Soulier syndrome (a giant platelet disorder) and von Willebrand disease.
• Post-translational modifications such as sulfation and phosphorylation regulate CD42b activity and stability.
• CD42b localizes to the plasma membrane as a type I transmembrane protein and is specifically expressed on platelets and megakaryocytes.
• It is involved in additional functions like thrombin-dependent platelet activation, contributing to coagulation.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the antibody is expected to detect a band at approximately 72 kDa in human platelet lysates. Consider using a reducing agent to resolve the protein completely.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) may be required for optimal staining on formalin-fixed paraffin-embedded tissues. Validate antibody specificity with appropriate isotype controls.
• For ELISA, the immunogen covering the extracellular domain suggests potential suitability as a capture or detection antibody, but pairing with a matched pair should be tested empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to high-quality IVD raw materials. Our portfolio includes validated monoclonal antibodies like this CD42b clone, optimized for hemostasis and platelet function research. Beyond single reagents, we supply bulk ancillary materials, antibody pairs, and can source rare or custom targets, ensuring a seamless transition from assay development to clinical application.