CD34 Rabbit pAb for WB - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a single-pass transmembrane glycoprotein originally identified as a hematopoietic progenitor cell antigen.
• It functions as a potential adhesion molecule that mediates attachment of hematopoietic stem cells to the bone marrow extracellular matrix or directly to stromal cells.
• CD34 may serve as a scaffold for lineage-specific glycans, thereby facilitating stem cell binding to lectins expressed by stromal cells or other marrow components.
• The protein presents carbohydrate ligands to selectins, suggesting a role in cell trafficking and homing.
• CD34 is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
• Subcellular localization is at the membrane, consistent with its adhesive functions.
• Post-translational modifications include extensive glycosylation and phosphorylation; the protein is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 32–290), so the antibody is likely to recognize surface-expressed CD34; consider its use for detecting native protein conformations.
• In Western blotting, a band around 41 kDa is expected, but glycosylation may cause upward shifts; validate antibody specificity using appropriate controls in your sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), antigen retrieval protocols may be necessary; optimization on positive and negative tissue controls is recommended.
• ELISA applications may benefit from titration to determine optimal coating concentrations; pairing with a validated detection system is advised.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supplies diagnostic manufacturers and research laboratories with high-quality polyclonal and monoclonal antibodies, including validated tools for hematopoiesis and stem cell biology. We offer bulk ancillary reagents and expert sourcing of rare targets, ensuring seamless procurement from concept to clinic. Contact us to discuss custom antibody development or scale-up requirements for your diagnostic workflow.