CD31/PECAM1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P16284

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• Cell adhesion molecule required for leukocyte transendothelial migration (TEM) under most inflammatory conditions; Tyr-690 phosphorylation is critical for TEM and lateral border recycling compartment trafficking. Related references: PMID:17580308 PMID:19342684
• Trans-homophilic interaction plays a role in endothelial cell-cell adhesion via cell junctions. Related references: PMID:27958302
• Heterophilic interaction with CD177 facilitates transendothelial migration of neutrophils. Related references: PMID:17580308
• Homophilic ligation prevents macrophage-mediated phagocytosis of viable leukocytes and promotes phagocytosis of apoptotic leukocytes. Related references: PMID:12110892
• Modulates bradykinin receptor BDKRB2 activation and regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Related references: PMID:18672896
• Induces susceptibility to atherosclerosis (By similarity).
• Does not protect against apoptosis.
• Localized to cell membrane, membrane raft, and cell junctions.
• Expressed on platelets, leukocytes, and endothelial cells; isoform Long predominates, while Delta12, Delta14-15, Delta14, and Delta15 show tissue-specific distribution.
• Contains immunoglobulin domains, disulfide bonds, glycosylation, palmitoylation, and phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen targets the cytoplasmic tail (aa 630-738), which may offer good accessibility in whole-cell lysates for western blotting. Consider using reducing conditions and validate band size.
• For immunofluorescence on paraffin-embedded sections, antigen retrieval may be necessary; optimize for your tissue type and fixation conditions.
• Expected protein weight is 83kDa; slight mobility shifts may occur due to post-translational modifications; include positive controls from human, mouse, or rat endothelial or leukocyte lysates.
• For ELISA, perform chessboard titrations to determine optimal coating antigen and antibody concentrations.
• Cross-reactivity with mouse and rat has been reported; confirm specificity in your experimental system using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials for CD31/PECAM1 research and diagnostic development. From validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents, we support assay developers in cardiovascular and inflammation applications. Our expertise also covers rare target raw-material sourcing, helping you streamline procurement from concept to clinic.