CD2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P06729

Core Product Specifications and Parameters

Biological Background: T-cell surface antigen CD2 Function and Localization

• CD2 is a T-cell surface antigen also known as erythrocyte receptor, LFA-2, LFA-3 receptor, rosette receptor, and T11/Leu-5.
• It mediates adhesion between T-cells and other cell types by interacting with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1.
• The cytoplasmic domain of CD2 is involved in T-cell triggering and signaling, playing a key role in T-cell activation.
• CD2 is a member of the immunoglobulin superfamily and contains immunoglobulin-like domains and disulfide bonds.
• It is a transmembrane glycoprotein localized at the cell membrane.
• CD2 is expressed in natural killer cells, as confirmed at the protein level.
• The protein is subject to post-translational modifications including glycosylation, which may affect its function.
• CD2 is implicated in cell adhesion and immune response processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic domain (amino acids 235-351), making this antibody suitable for detecting total CD2 protein, including both membrane-bound and signaling forms.
• For Western blot (WB), consider validating the antibody in relevant human T-cell or NK cell lines. A band around the predicted molecular weight of 39 kDa (theoretical) may be observed.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval may be necessary; validate the protocol in positive control tissues such as lymphoid tissues or T-cell lymphomas.
• For ELISA, optimize coating antigen and antibody dilutions using recombinant human CD2 or cell lysates as capture targets.
• Since CD2 is a glycoprotein, deglycosylation enzymes may be used to confirm specificity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing validated antibodies, antibody pairs, and bulk reagents. This CD2 antibody supports T-cell adhesion and signaling studies, and we offer complementary ancillary reagents and custom sourcing for your IVD development workflows.