CD19 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P15391

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 is a B-lymphocyte surface antigen (also known as B4, CVID3) that functions as a coreceptor for the B-cell antigen receptor (BCR) complex, critical for modulating B-cell activation thresholds. Related references: PMID:29523808
• It decreases the threshold for downstream signaling and B-cell responses to antigens, enhancing sensitivity. Related references: PMID:1373518, PMID:16672701, PMID:2463100
• CD19 activates phosphatidylinositol 3-kinase (PI3K) and intracellular Ca2+ mobilization, essential for B-cell signaling cascades. Related references: PMID:12387743, PMID:16672701, PMID:9317126
• While not required for early B-cell development in bone marrow, CD19 is necessary for normal B-cell differentiation and proliferation upon antigen challenge. Related references: PMID:9317126, PMID:1373518, PMID:2463100
• It is required for normal serum immunoglobulin levels and production of high-affinity antibodies, highlighting its role in humoral immunity. Related references: PMID:12387743, PMID:16672701, PMID:9317126
• CD19 is detected on marginal zone and germinal center B cells in lymph nodes and on peripheral blood B cells at the protein level. Related references: PMID:2463100, PMID:16672701
• It is a transmembrane glycoprotein localized to the cell membrane and membrane rafts, with an extracellular immunoglobulin-like domain and multiple post-translational modifications including phosphorylation and N-glycosylation.
• CD19 is involved in adaptive immunity and serves as a B-cell lineage marker, with implications in immunodeficiency disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal cytoplasmic region (aa 457-556) of human CD19. Verify reactivity with full-length native protein in your assay system.
• For Western blotting, expect a specific band near 61 kDa. Optimize loading amounts and transfer conditions for clear resolution.
• In IHC-P, antigen retrieval (e.g., heat-induced epitope retrieval) may enhance epitope accessibility. Titrate antibody to determine optimal working dilution.
• For IF-P, permeabilization is required to detect the intracellular epitope. Test fixation and permeabilization protocols compatible with your sample.
• Cross-reactivity with cynomolgus monkey supports potential use in non-human primate models. Validate in the relevant sample system.
• For ELISA, consider a direct or capture format and include a recombinant CD19 standard curve for quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials and technical support. Our CD19 monoclonal antibody is part of a comprehensive portfolio of B-cell markers and immunological reagents, supporting assay development across diverse platforms. We supply validated antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your project from concept to clinic.