CD172a/SIRPα Rabbit mAb for WB, IF/ICC, ELISA - P78324

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPα) Function and Localization

• Encoded by the SIRPA gene, this protein is a member of the signal-regulatory protein family and is also known as SHPS1, CD172a, BIT, MFR, MYD-1, and p84.
• Functions as a cell surface receptor for CD47, mediating inhibitory signals that negatively regulate phagocytosis, mast cell activation, and dendritic cell maturation.
• Also acts as a receptor for thrombospondin-1 (THBS1), stimulating SIRPA phosphorylation and NADPH oxidase-derived ROS production in non-phagocytic cells. Related references: PMID:24511121
• Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses triggered by cell adhesion, growth factors, or insulin.
• Plays a role in neuronal processes, including adhesion of cerebellar neurons, neurite outgrowth, and glial cell attachment, and may contribute to synaptic function.
• Localized to the plasma membrane and is ubiquitously expressed, with highest levels in brain and on myeloid cells; lower levels in heart, placenta, lung, liver, and other tissues.
• Subject to alternative splicing and post-translational modifications including glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 395–495 within the cytoplasmic domain, suggesting the antibody recognizes an intracellular epitope; permeabilization is likely required for IF/ICC detection.
• For western blot, a band near 55 kDa is expected; however, glycosylation and other modifications may cause slight shifts.
• Cross-reactivity with mouse and rat has been reported, making this antibody suitable for comparative studies across these species; validation in the specific experimental system is recommended.
• As a rabbit monoclonal antibody, it offers high affinity and lot-to-lot consistency; optimal dilutions should be determined empirically for each application.

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