CD160 Rabbit pAb for WB and ELISA - O95971

Core Product Specifications and Parameters

Biological Background: CD160 Function and Localization

• CD160 is a receptor on immune cells that exists in both GPI-anchored and transmembrane forms, mediating stimulatory or inhibitory signals through PI3K in NK cells and through LCK/CD247 in T cells. Related references: PMID:11978774 · PMID:17307798 · PMID:19109136
• It functions as a receptor for classical and non-classical MHC class I molecules. In acute viral infection, it recognizes HLA-C to trigger NK cell cytotoxicity; on CD8+ T cells, it binds HLA-A2-B2M/viral peptide complexes to provide costimulation. Related references: PMID:12486241 · PMID:9973372
• During chronic viral infection, persistent antigen stimulation may cause CD160 to progressively inhibit TCR signaling in memory CD8+ T cells, contributing to T cell exhaustion. Related references: PMID:25255144
• On endothelial cells, CD160 recognizes HLA-G and controls angiogenesis in immune-privileged sites. Related references: PMID:16809620
• CD160 engages in bidirectional signaling with TNFRSF14: it down-regulates CD28 costimulation on activated CD4+ T cells (restricting memory/alloantigen responses) and provides stimulatory signals to NK cells (by similarity). Related references: PMID:18193050
• The protein is expressed predominantly in NK cells, cytotoxic T lymphocytes (including both alpha-beta and gamma-delta subsets), memory/effector CD8+ T cells, endothelial cells, and at lower levels in monocytes and B cells. A specific isoform (isoform 3) is exclusive to activated NK cells. Related references: PMID:11978774 · PMID:18193050 · PMID:25255144 · PMID:9743336 · PMID:16809620 · PMID:23761635 · PMID:19109136
• Structurally, CD160 is a GPI-anchored glycoprotein with an immunoglobulin domain and disulfide bonds; a soluble form can be released by activated lymphocytes to potentially limit effector functions.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal extracellular region (residues 1-181) of human CD160, suggesting recognition of the ligand-binding domain. When performing WB, consider that the ~20 kDa band may represent the unglycosylated backbone; mature GPI-anchored and soluble forms can exhibit higher molecular weights due to glycosylation and anchor modifications.
• For ELISA applications, the antibody may detect both membrane-bound and soluble CD160; optimizing the coating format and using appropriate recombinant standards is recommended.
• Cross-reactivity with mouse and rat has been reported, but epitope conservation should be verified in the experimental system of interest—especially if using genetically modified models.
• As a polyclonal antibody, lot-to-lot consistency should be assessed with positive and negative control lysates or recombinant protein; include appropriate secondary antibody controls.
• For functional studies, note that CD160 can mediate both costimulatory and inhibitory outcomes depending on the cellular context; parallel readouts of immune activation markers are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop platform for IVD raw materials, empowering diagnostic developers with validated antibodies, optimized pairs, and bulk ancillary reagents. This CD160 polyclonal antibody exemplifies our capability to supply research-use reagents for immune checkpoint and lymphocyte receptor studies. Beyond individual catalog items, we offer tailored solutions—from rare-target antibody generation to conjugated monoclonals and custom antigen production—supporting your entire assay development pipeline from proof-of-concept to scalable manufacturing.