CD14 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14, also known as My23 antigen, is a myeloid cell-specific leucine-rich glycoprotein that serves as a monocyte differentiation antigen.
• Functions as a coreceptor for bacterial lipopolysaccharide (LPS), partnering with LBP to bind monomeric LPS and deliver it to the TLR4/LY96 complex, thereby mediating the innate immune response. Related references: PMID:1698311 PMID:23264655 PMID:20133493 PMID:22265692
• Acts via MyD88, TIRAP, and TRAF6, leading to NF-kappa-B activation and cytokine secretion. Related references: PMID:8612135
• Also functions as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, with signaling from the cell surface and subsequent trafficking to the Golgi in a lipid-raft dependent pathway. Related references: PMID:16880211
• Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). Related references: PMID:23880187
• Localized to the cell membrane, secreted form exists, and is found in membrane rafts and the Golgi apparatus.
• Expressed strongly on the surface of monocytes and weakly on granulocytes; also expressed by most tissue macrophages. Related references: PMID:1698311
• Contains leucine-rich repeats, is glycosylated, and anchored to the membrane via a GPI anchor.

Experimental Guidance and Technical Tips

• For Western blot, a reduced band around 40 kDa is expected; consider validating in lysates from monocytes or macrophages.
• For immunofluorescence, CD14 localizes to the cell membrane and Golgi; optimize permeabilization and fixation conditions for your sample.
• ELISA applications may be suitable for detecting soluble CD14 (sCD14) in serum or plasma; appropriate standards and controls should be included.
• As a polyclonal antibody, verify lot-to-lot consistency and validate in the relevant sample system before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with one-stop access to high-quality IVD raw materials, including validated CD14 antibodies for innate immunity studies. From monoclonal and polyclonal antibodies to ancillary reagents and bulk sourcing, our comprehensive supply chain supports every stage of your assay development—from target validation to clinical application.