CD11b/ITGAM Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P11215

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M Function and Localization

• Integrin alpha-M (ITGAM), also designated CD11b, CR3A, MO1A, or MAC-1, is the alpha subunit of the leukocyte-specific integrin MAC-1 (αMβ2, CD11b/CD18). Related references: PMID:20008295, PMID:9558116
• It functions as a receptor for the iC3b fragment of complement component 3, facilitating the uptake of complement-coated particles and pathogens. Related references: PMID:20008295, PMID:9558116
• ITGAM/ITGB2 heterodimer also acts as a receptor for fibrinogen and factor X, recognizing specific peptides in the fibrinogen gamma chain.
• Regulates neutrophil migration. Related references: PMID:28807980
• In association with the beta subunit ITGB2 (CD18), ITGAM is required for CD177-PRTN3-mediated activation of TNF-primed neutrophils. Related references: PMID:21193407
• Serves as a receptor for ICAM1, mediating adhesion between stimulated neutrophils and endothelial cells. Related references: PMID:1980124
• Predominantly expressed in monocytes and granulocytes; protein expression confirmed in neutrophils. Related references: PMID:1346576, PMID:21193407
• Localizes to the cell membrane and membrane rafts. May also play roles in phagocytosis-induced apoptosis, mast cell development, and microglial superoxide production during brain development (By similarity).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 145–340 of CD11b/ITGAM, an extracellular region likely exposed on the native protein; consider this when designing detection strategies.
• For Western blot (WB), a band around 127 kDa is anticipated; run appropriate molecular weight markers and validate with relevant positive controls (e.g., monocyte or granulocyte lysates).
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), membrane localization is expected; optimize fixation and permeabilization conditions to preserve epitope accessibility.
• The antibody has been reported to cross-react with mouse and rat ITGAM; however, sequence homology in the immunogen region should be verified for your specific model organism.
• ELISA applications may benefit from using the recombinant immunogen or native integrin preparations as capture antigens; titrate antibody concentrations accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies, bulk ancillary reagents, and rare-target sourcing capabilities. The CD11b/ITGAM antibody supports research into innate immunity, cell adhesion, and inflammatory diseases. As your one-stop sourcing partner, CamelBio streamlines procurement from concept to commercial production, ensuring consistent supply and technical support.