CD11b Rabbit PolymAb® Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P11215 / P05555

Core Product Specifications and Parameters

Biological Background: ITGAM (CD11b) Function and Localization

• ITGAM encodes integrin alpha-M (CD11b), a transmembrane receptor that pairs with integrin beta-2 (CD18) to form the Mac-1 (CR3) complex, a key adhesion molecule in innate immunity.
• Functions as a complement receptor by binding the iC3b fragment of C3, mediating phagocytosis of complement-opsonized pathogens (PubMed:20008295, PubMed:9558116). Related references: PMID:20008295 PMID:9558116
• Mediates adhesion of neutrophils to stimulated endothelial cells via ICAM1 interaction, facilitating leukocyte extravasation and migration (PubMed:1980124). Related references: PMID:1980124
• Required with CD18 for CD177-PRTN3-mediated activation of TNF-primed neutrophils, amplifying inflammatory responses (PubMed:21193407). Related references: PMID:21193407
• Regulates neutrophil migration and contributes to innate immune defense (PubMed:28807980). Related references: PMID:28807980
• Subcellularly localizes to the cell membrane and membrane rafts, where it interacts with signaling molecules.
• Predominantly expressed in monocytes, granulocytes, and neutrophils, consistent with its myeloid-restricted function.
• Undergoes alternative splicing and extensive N-glycosylation; contains multiple calcium- and magnesium-binding repeats essential for activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–100 amino acids of human CD11b; antibody binding may be affected if the epitope map lies within this region or if post-translational modifications alter accessibility.
• For Western blotting (WB) under reducing conditions, a band at approximately 127 kDa is anticipated; using a high molecular weight marker and adequate protein transfer time is advisable.
• When performing IHC-P analysis, test different antigen retrieval methods (e.g., citrate buffer, pH 6.0) and primary antibody dilutions to optimize nuclear/membrane-specific signals.
• In IF/ICC applications, consider cell permeabilization protocols and fixation methods that preserve membrane antigen integrity; confirm localization aligns with expected plasma membrane staining.
• The antibody cross-reacts with mouse and rat CD11b; however, always validate in the relevant sample system to ensure specificity.
• For ELISA, this antibody could be evaluated as a capture antibody in sandwich pairings targeting CD11b; assess performance with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with validated antibody reagents and supporting components. For CD11b-related immunology and inflammation research, our portfolio includes high-quality monoclonal antibodies, antigen pairs, and ancillary reagents, ensuring reliable supply and technical backing from concept to clinical application.