CD11b (Integrin alpha-M) Rabbit Polyclonal Antibody for WB and ELISA - P11215

Core Product Specifications and Parameters

Biological Background: CD11b Function and Localization

• Integrin alpha-M (CD11b) pairs with the beta-2 subunit (ITGB2/CD18) to mediate adhesive interactions and uptake of complement-coated particles and pathogens in monocytes, macrophages, and granulocytes.
  Related references: PMID:20008295, PMID:9558116

• It functions as the receptor CR-3 for the iC3b fragment of complement component C3, likely recognizing the R-G-D peptide sequence, and also binds fibrinogen, factor X, and ICAM-1 to support leukocyte adhesion.

• CD11b regulates neutrophil migration and, in complex with CD18, is required for CD177-PRTN3-mediated activation of TNF-primed neutrophils.
  Related references: PMID:28807980, PMID:21193407

• Interaction with endothelial ICAM-1 promotes adhesion between stimulated neutrophils and endothelial cells.
  Related references: PMID:1980124

• In microglia, CD11b cooperates with TYROBP/DAP12 to regulate superoxide production and neuronal apoptosis during brain development.

• Predominantly expressed in monocytes, granulocytes, and neutrophils, CD11b localizes to the cell membrane and membrane rafts.

• The protein contains integrin-type repeats, disulfide bonds, and glycosylation sites, and undergoes alternative splicing, giving rise to multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1-100); the resulting polyclonal antibody may recognize linear epitopes, making it suitable for Western blot under denaturing conditions.
• For Western blot, the predicted molecular weight of CD11b is ~127 kDa; however, glycosylation may cause band shifts in cell lysates. Validate with appropriate positive controls.
• In ELISA applications, polyclonal antibodies can provide high sensitivity; consider pairing with a monoclonal detection antibody for sandwich ELISA development.
• Cross-reactivity with mouse and rat CD11b suggests epitope conservation; verify species-specific reactivity in your experimental system if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. This CD11b polyclonal antibody serves immunology and inflammation research, complementing our portfolio of validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For specialized targets like rare leukocyte antigens, our team can assist in sourcing custom raw materials to accelerate your assay development.